Fast Green FCF  (Synonyms: 固绿 FCF; FD&C Green No. 3; Food green 3; C.I. 42053)

Fast Green FCF is a sea green triarylmethane food dye, with absorption maximum ranging from 622 to 626 nm. Fast Green FCF inhibits α-synuclein aggregation, as well as Aβ and P2X4 receptor, and TLR4/Myd88/NF-κB. Fast Green FCF is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function^{[1][2][3][4][5][6][7][8][9]}.

Fast Green FCF (HEWL/FCF 摩尔比：1 比 0.01-1 比 1，10 天) 以浓度依赖性方式抑制溶菌酶原纤维的生成，并对预先形成/存在的溶菌酶原纤维表现出分解活性^[2]。
Fast Green FCF (100 μg/mL-1 mg/mL) 抑制大鼠海马中间神经元的突触活动，半最大有效浓度约为 200 μg/mL (250 μM)^[3]。
Fast Green FCF (5-100 μM，24 h) 抑制 α-突触核蛋白聚集，分解成熟原纤维并防止 PC12 细胞中淀粉样蛋白诱导的神经毒性^[4]。
Fast Green FCF (Aβ40 与 FGF 的摩尔比为 3:1-1:3) 可减轻 Aβ40 纤维形成引起的 PC12 细胞毒性^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Fast Green FCF (100 mg/kg，腹腔注射，每次试验前 30 min) 可减轻小鼠 Aβ 诱导的认知障碍^[5]。 Fast Green FCF (10-100 mg/kg，腹腔注射，每日一次，手术前 30 min) 通过下调小鼠 P2X4 受体来预防术后认知功能障碍^[6]。 Fast Green FCF (100 mg/kg，腹腔注射，每日一次，连续 7 天) 可减轻脂多糖诱导的抑郁样行为，并下调小鼠海马中的 TLR4/Myd88/NF-κB 信号通路^[7]。 Fast Green FCF (30-100 mg/kg，腹腔注射，每日一次，共 9 天) 可缓解疼痛过敏，并下调啮齿动物炎症疼痛模型中的脊髓 P2X4 表达和促炎细胞因子水平^[8]。 Fast Green FCF (0.017-0.041 g/Kg 体重，口服，42 天) 可导致睾丸重量、管状直径、精子密度和精子活力显著下降^[9]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

808.85

C₃₇H₃₄N₂Na₂O₁₀S₃

2353-45-9

固体

Dark green to black

522

486

固绿 FCF； 坚牢绿 FCF； 快绿

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

• [1]. Mittal A, et al. Batch and bulk removal of a triarylmethane dye, Fast Green FCF, from wastewater by adsorption over waste materials. J Hazard Mater. 2009 Apr 30;163(2-3):568-77.  [Content Brief]

  [2]. How SC, et al. Examining the inhibitory potency of food additive fast green FCF against amyloid fibrillogenesis under acidic conditions. Food Funct. 2016 Dec 7;7(12):4898-4907.  [Content Brief]

  [3]. van Hooft JA. Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons. Neurosci Lett. 2002 Feb 1;318(3):163-5.  [Content Brief]

  [4]. Wang F, et al. The food additive fast green FCF inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity. Food Funct. 2021 Jun 21;12(12):5465-5477.  [Content Brief]

  [5]. Liu F, et al. Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice. Int J Biol Macromol. 2021 Feb 15;170:33-41.  [Content Brief]

  [6]. Liu J, et al. Fast green FCF prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice. Int Immunopharmacol. 2023 Aug;121:110462.  [Content Brief]

  [7]. Yang J, et al. Fast Green FCF Attenuates Lipopolysaccharide-Induced Depressive-Like Behavior and Downregulates TLR4/Myd88/NF-κB Signal Pathway in the Mouse Hippocampus. Front Pharmacol. 2019 May 8;10:501.  [Content Brief]

  [8]. Xu F, et al. Fast Green FCF Alleviates Pain Hypersensitivity and Down-Regulates the Levels of Spinal P2X4 Expression and Pro-inflammatory Cytokines in a Rodent Inflammatory Pain Model. Front Pharmacol. 2018 May 23;9:534.  [Content Brief]

  [9]. Dixit A, et al. ASSESSMENT OF REPRODUCTIVE TOXICITY CAUSED BY FAST GREEN FCF ON MALE SWISS ALBINO MICE. 2012.