INH1 (Synonyms: IBT13131)

目录号: HY-16660 纯度: 99.85%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

INH1 通过直接结合 Hec1，特异性干扰 Hec1/Nek2 的相互作用。INH1 在体内和体外均表现出良好的抗肿瘤活性。

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INH1 Chemical Structure

CAS No. : 313553-47-8

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可免费申领三个不同产品的试用装。

3. 试用装只面向终端客户。

规格	价格	是否有货
Free Sample (0.1 - 0.2 mg)		Apply now
10 mM * 1 mL in DMSO	￥330	In-stock
5 mg	￥300	In-stock
10 mg	￥500	In-stock
25 mg	￥1100	In-stock
50 mg	￥1800	In-stock
100 mg	￥2900	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

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INH1 相关产品

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NEK1 NEK2 NEK6 NEK7

生物活性
纯度 & 产品资料
参考文献

生物活性

INH1 specifically disrupts the Hec1/Nek2 interaction via direct Hec1 binding. INH1 shows promising cancer inhibition activity both in vitro and in vivo^[1].

IC₅₀ & Target

NEK2

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	8.8 μM Compound: INH1	Cytotoxicity against human HeLa cells after 4 days by XTT assay Cytotoxicity against human HeLa cells after 4 days by XTT assay	[PMID: 19243176]
K562	IC₅₀	11.7 μM Compound: INH1	Cytotoxicity against human K562 cells after 4 days by XTT assay Cytotoxicity against human K562 cells after 4 days by XTT assay	[PMID: 19243176]
MDA-MB-231	IC₅₀	0.839 μM Compound: 3o	Inhibition of cell migration in human MDA-MB-231 cells assessed as total number of cells after 24 hrs by transwell migration assay Inhibition of cell migration in human MDA-MB-231 cells assessed as total number of cells after 24 hrs by transwell migration assay	[PMID: 23526571]
MDA-MB-231	IC₅₀	8.6 μM Compound: INH1	Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay	[PMID: 19243176]
MDA-MB-468	IC₅₀	10.5 μM Compound: INH1	Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay	[PMID: 19243176]

体外研究
(In Vitro)

INH1 (25 μM, 24 h) treatment resulted in reduced association of Hec1 with kinetochore and decrease of global Nek2 protein level^[1].
INH1 exhibits GI₅₀ values of 10.5 μM (in MDA-MB-468 cells), 15 μM (in SKBR3 cells), 10.5 μM (in T47D cells), 20.5 μM (in MDA-MB-361 cells), 15 μM (in ZR-75-1 cells), 15 μM (in HBL 100 cells), 15.5 μM (in MDA-MB-435 cells), 11 μM (in HS578T cells) and 41 μM (in MCF10A cells), respectively^[1].
INH1 (5k) has an IC₅₀ value of 176 nM in the dose-dependent transwell migration assays in MDA-MB-231 cells. INH1 (5k) substantially reduces cellular f-actin and prevented localization of fascin to actin-rich membrane protrusions^[2].
INH1 induces abnormal mitotic processes, as well as cell apoptosis^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	MCF10A cells.
Concentration:	10 μM.
Incubation Time:	12 days.
Result:	Effectively inhibits the proliferation of human breast cancer lines.

Western Blot Analysis^[1]

Cell Line:	MCF10A cells.
Concentration:	25 μM.
Incubation Time:	24 h.
Result:	Nek2 reduction in INH1-treated cells may be independent of Hec1.

体内研究
(In Vivo)

INH1 (50 or 100 mg/kg, i.p., every other day/25 cycles) inhibits tumor outgrowth in a xenografted breast cancer model in nude mice^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenografted nude mice breast cancer model^[1].
Dosage:	50 or 100 mg/kg.
Administration:	I.P., every other day/25 cycles.
Result:	Inhibited tumor growth.

分子量

308.40

Formula

C₁₈H₁₆N₂OS

CAS 号

313553-47-8

性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 100 mg/mL (324.25 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.2425 mL	16.2127 mL	32.4254 mL
5 mM	0.6485 mL	3.2425 mL	6.4851 mL
10 mM	0.3243 mL	1.6213 mL	3.2425 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： 17% Solutol HS-15 in Saline
Solubility: 20 mg/mL (64.85 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: 99.85%

选择批次：

Data Sheet (617 KB) SDS (393 KB)

COA (286 KB) HNMR (264 KB) LCMS (96 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Wu G, et al. Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal. Cancer Res. 2008 Oct 15;68(20):8393-9. [Content Brief]

[2]. Shilong Zheng, et al. Discovery of a Series of Thiazole Derivatives as Novel Inhibitors of Metastatic Cancer Cell Migration and Invasion. ACS Med Chem Lett. 2013 Feb 14; 4(2): 191–196. [Content Brief]

[3]. Yongxia Zhu, et al. Small Molecule TH-39 Potentially Targets Hec1/Nek2 Interaction and Exhibits Antitumor Efficacy in K562 Cells via G0/G1 Cell Cycle Arrest and Apoptosis Induction. Cell Physiol Biochem. 2016;40(1-2):297-308. [Content Brief]

INH1 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2425 mL	16.2127 mL	32.4254 mL	81.0636 mL
	5 mM	0.6485 mL	3.2425 mL	6.4851 mL	16.2127 mL
	10 mM	0.3243 mL	1.6213 mL	3.2425 mL	8.1064 mL
	15 mM	0.2162 mL	1.0808 mL	2.1617 mL	5.4042 mL
	20 mM	0.1621 mL	0.8106 mL	1.6213 mL	4.0532 mL
	25 mM	0.1297 mL	0.6485 mL	1.2970 mL	3.2425 mL
	30 mM	0.1081 mL	0.5404 mL	1.0808 mL	2.7021 mL
	40 mM	0.0811 mL	0.4053 mL	0.8106 mL	2.0266 mL
	50 mM	0.0649 mL	0.3243 mL	0.6485 mL	1.6213 mL
	60 mM	0.0540 mL	0.2702 mL	0.5404 mL	1.3511 mL
	80 mM	0.0405 mL	0.2027 mL	0.4053 mL	1.0133 mL
	100 mM	0.0324 mL	0.1621 mL	0.3243 mL	0.8106 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

INH1313553-47-8IBT13131INH 1INH-1IBT 13131IBT-13131NEKsApoptosisNIMA–related KinasesNIMA (never in mitosis gene a)-related Expressed KinasesInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

INH1 (Synonyms: IBT13131)

Customer Review