2. Stem Cell/Wnt
  3. Wnt
  4. IWP-4

IWP-4 是一种小分子 Wnt 抑制剂，其 IC50 值为 25 nM。

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IWP-4 Chemical Structure

IWP-4 Chemical Structure

CAS No. : 686772-17-8

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规格 价格 是否有货 数量
1 mg ￥438
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5 mg ￥1500
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10 mg ￥2500
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25 mg ￥5000
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Customer Review

IWP-4 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

IWP-4 is a small molecule Wnt inhibitor with an IC50 of 25 nM.

IC50 & Target

IC50: 25 nM (Wnt)[1]
细胞效力
(Cellular Effect)
Cell Line Type Value Description References
CAPAN-1 EC50
0.23 μM
Compound: IWP-4
Antiproliferative activity against human Capan1 cells after 48 hrs by MTT assay
Antiproliferative activity against human Capan1 cells after 48 hrs by MTT assay
[PMID: 29630366]
HEK293 EC50
0.28 μM
Compound: IWP-4
Antiproliferative activity against human HEK293 cells after 48 hrs by MTT assay
Antiproliferative activity against human HEK293 cells after 48 hrs by MTT assay
[PMID: 29630366]
HT-29 EC50
0.34 μM
Compound: IWP-4
Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay
Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay
[PMID: 29630366]
MIA PaCa-2 EC50
0.23 μM
Compound: IWP-4
Antiproliferative activity against human MIAPaCa2 cells after 48 hrs by MTT assay
Antiproliferative activity against human MIAPaCa2 cells after 48 hrs by MTT assay
[PMID: 29630366]
PANC-1 EC50
0.23 μM
Compound: IWP-4
Antiproliferative activity against human PANC1 cells after 48 hrs by MTT assay
Antiproliferative activity against human PANC1 cells after 48 hrs by MTT assay
[PMID: 29630366]
SW-620 EC50
0.23 μM
Compound: IWP-4
Antiproliferative activity against human SW620 cells after 48 hrs by MTT assay
Antiproliferative activity against human SW620 cells after 48 hrs by MTT assay
[PMID: 29630366]
体外研究
(In Vitro)

IWP-4 是一种小分子Wnt抑制剂，其IC50值为25 nM。IWP-4能够促进心肌标志物的表达，包括心肌肌钙蛋白I (CTNI) 和心肌肌球蛋白重链亮细胞 (MYHhi+)。此外，IWP-4还能导致跳动焦点的出现（0.44±0.10 SEM 次/秒），这种现象在未接受IWP-4处理的所有培养物中均不存在。进一步地，流式细胞术分析表明，在第16天时，IWP-4处理过的培养物中存在明显更多的MYHlo+细胞 (P<0.0002)，分别为17.0±1.3 SD% 和未经处理的5.4±1.4 SD%。NKX2-5蛋白表达量的量化显示，63% (481/817) 的IWP-4处理细胞显示出核内NKX2-5的表达[1]。
间充质前体细胞(MPCs)经过IWP-4处理后，在第7天与仅使用成骨诱导培养基相比，*AXIN2*、*CTNNB1* 和 *GSK3B* 的表达没有显著变化；但在第21天时，IWP-4处理的MPCs中*DKK1* 和 *GSK3β* 表达水平有所升高。同时，IWP-4也引起了*SPARC* 和 *COL1A1* 的显著下调[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IWP-4 相关抗体:
分子量

496.62

Formula

C23H20N4O3S3
CAS 号
性状

固体

颜色

White to light yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 1.3 mg/mL (2.62 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0136 mL 10.0681 mL 20.1361 mL
5 mM --- --- ---
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.44%

COA (287 KB) HNMR (261 KB) RP-HPLC (271 KB) MS (68 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[1]

hESC cultures are obtained in mTeSR-1 medium and expanded with daily medium exchange until colonies reach the desired level of confluence (~70% to 80%). At this time (marked day 0), mTeSR-1 is replaced with a basal medium comprised of RPMI 1640 medium supplemented with 2% B27 supplement and 1% penicillin/streptomycin. 20 ng/mL BMP-4 and/or 6 ng/mL activin A are added to the basal medium for primitive streak induction, and exchanged daily until day 3. Then, basal media with or without 5 mM IWP-4 is added to the cells and exchanged every 2 days [dimethyl sulfoxide (DMSO) at the same concentration is used as a vehicle control] until day 15, after which basal medium is supplied every 2 days[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

IWP-4 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0136 mL 10.0681 mL 20.1361 mL 50.3403 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IWP-4686772-17-8IWP4IWP 4WntInhibitorinhibitorinhibit

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目录号：
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