M344 (Synonyms: D 237; MS 344)

目录号: HY-13506 纯度: 98.86%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

M344 (D 237) 是一种组蛋白去乙酰化酶抑制剂，IC₅₀ 为 100 nM。

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M344 Chemical Structure

CAS No. : 251456-60-7

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥891	In-stock
5 mg	￥810	In-stock
10 mg	￥1265	In-stock
50 mg	￥3800	In-stock
100 mg		询价
200 mg		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

Other Forms of M344:

M344 (GMP) 询价

MCE 顾客使用本产品发表的 2 篇科研文献

M344 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 HDAC 亚型特异性产品:

查看所有亚型

HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

M344 (D 237) is an inhibitor of histone deacetylase (IC₅₀=100 nM) and an inducer of terminal cell fifferentiation.

IC₅₀ & Target^[1]

HDAC

100 nM (IC₅₀)

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-431	IC₅₀	2.3 μM Compound: 23	Concentration required to inhibit the epidermoid A431 cell growth by 50%, P53 status of cell line used was wild-type. Concentration required to inhibit the epidermoid A431 cell growth by 50%, P53 status of cell line used was wild-type.	[PMID: 11831887]
A549	IC₅₀	0.8 μM Compound: 23	Concentration required to inhibit the lung A549 cell growth by 50%,P53 status of cell line used was wild-type. Concentration required to inhibit the lung A549 cell growth by 50%,P53 status of cell line used was wild-type.	[PMID: 11831887]
HCT-116	IC₅₀	0.2 μM Compound: 23	Concentration required to inhibit the colon HCT116 cell growth by 50%,P53 status of cell line used was wild-type. Concentration required to inhibit the colon HCT116 cell growth by 50%,P53 status of cell line used was wild-type.	[PMID: 11831887]
HCT-116	IC₅₀	0.21 μM Compound: 23	Concentration required to inhibit the HCT116 cell growth by 50%. Concentration required to inhibit the HCT116 cell growth by 50%.	[PMID: 11831887]
LS174T	IC₅₀	1.5 μM Compound: 23	Concentration required to inhibit the colon LS 174T cell growth by 50%,P53 status of cell line used was mutant-type. Concentration required to inhibit the colon LS 174T cell growth by 50%,P53 status of cell line used was mutant-type.	[PMID: 11831887]
NCI-H1299	IC₅₀	3.9 μM Compound: 23	Concentration required to inhibit the lung H1299 cell growth by 50%,P53 status of cell line used was wild-type. Concentration required to inhibit the lung H1299 cell growth by 50%,P53 status of cell line used was wild-type.	[PMID: 11831887]
SW-620	IC₅₀	0.7 μM Compound: 23	Concentration required to inhibit the colon SW 620 cell growth by 50%,P53 status of cell line used was mutant-type. Concentration required to inhibit the colon SW 620 cell growth by 50%,P53 status of cell line used was mutant-type.	[PMID: 11831887]
T-24	IC₅₀	3.8 μM Compound: 23	Concentration required to inhibit the bladder T24 cell growth by 50%,P53 status of cell line used was wild-type. Concentration required to inhibit the bladder T24 cell growth by 50%,P53 status of cell line used was wild-type.	[PMID: 11831887]

体外研究
(In Vitro)

M344 是一种潜在的组蛋白脱乙酰酶 (HDAC) 抑制剂。随着 M344 浓度的增加，BRCA1 mRNA 出现剂量依赖性降低，并且使用 1 μM 和 5 μM 浓度的 M344 处理导致所有检查的细胞系中 BRCA1 表达显著降低。与顺铂单独处理相比，M344 与顺铂联合导致所有细胞系中 BRCA1 mRNA 表达降低，A2780s 除外，A2780s 被认为对顺铂具有强细胞毒性。在 MCF7 细胞系中，BRCA1 在生理剂量的 M344 (0.5 μM 和 1 μM) 下调，但 M344 在 5.0 μM 剂量下对 BRCA1 没有相同的抑制作用。在所有检查的乳腺和卵巢细胞系中，与顺铂和增加浓度的 M344 联合处理可降低 BRCA1 蛋白水平^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

307.39

Formula

C₁₆H₂₅N₃O₃

CAS 号

251456-60-7

性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 100 mg/mL (325.32 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.2532 mL	16.2660 mL	32.5320 mL
5 mM	0.6506 mL	3.2532 mL	6.5064 mL
10 mM	0.3253 mL	1.6266 mL	3.2532 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.13 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.13 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.13 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 98.86%

选择批次：

Data Sheet (633 KB) SDS (393 KB)

COA (186 KB) LCMS (261 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Jung M1, et al. Amide analogues of trichostatin A as inhibitors of histone deacetylase and inducers of terminal cell differentiation. J Med Chem. 1999 Nov 4;42(22):4669-79. [Content Brief]

[2]. Weberpals JI, et al. The effect of the histone deacetylase inhibitor M344 on BRCA1 expression in breast and ovarian cancer cells. Cancer Cell Int. 2011 Aug 19;11(1):29. [Content Brief]

Cell Assay ^[2]	The A2780s and A2780cp cell lines, the T-47D and OVCAR-4 cell lines. and the MCF7 and HCC1937cell lines, are maintained in Dulbecco's-MEM supplemented with 10% fetal bovine serum and 100 μg/mL penicillin-streptomycin. Unless otherwise described, cells are treated for 24 hrs with 2 μg/mL Cisplatin alone, and in combination with the HDAC inhibitor M344 at concentrations of 0.5, 1.0, or 5.0 μM. Phase contrast images are collected using the 10× objective of an Eclipse TE2000-U. Cell viability is measured by the MTT rapid colorimetric assay. Approximately 4,500 cells are seeded into each well of a 96-well flat bottom plate. The cells are incubated overnight to allow for cell attachment. Cells are then treated with Cisplatin in concentrations of 0-8 μg/mL alone or in combination with 1 μM of the HDAC inhibitor, M344. Forty eight hours following treatment, 42 μL of a 5 mg/mL MTT substrate solution in phosphate buffered saline (PBS) is added and incubated for up to 4 hrs at 37°C. The resulting violet formazan precipitate is solubilized by the addition of 82 μL of a 0.01 M HCl/10% SDS solution and plates are incubated overnight at 37°C. The plates are then analyzed on an MRX Microplate Reader at 570 nm to determine the optical density of the samples^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Jung M1, et al. Amide analogues of trichostatin A as inhibitors of histone deacetylase and inducers of terminal cell differentiation. J Med Chem. 1999 Nov 4;42(22):4669-79. [Content Brief] [2]. Weberpals JI, et al. The effect of the histone deacetylase inhibitor M344 on BRCA1 expression in breast and ovarian cancer cells. Cancer Cell Int. 2011 Aug 19;11(1):29. [Content Brief]

Cell Assay
^[2]

The A2780s and A2780cp cell lines, the T-47D and OVCAR-4 cell lines. and the MCF7 and HCC1937cell lines, are maintained in Dulbecco's-MEM supplemented with 10% fetal bovine serum and 100 μg/mL penicillin-streptomycin. Unless otherwise described, cells are treated for 24 hrs with 2 μg/mL Cisplatin alone, and in combination with the HDAC inhibitor M344 at concentrations of 0.5, 1.0, or 5.0 μM. Phase contrast images are collected using the 10× objective of an Eclipse TE2000-U. Cell viability is measured by the MTT rapid colorimetric assay. Approximately 4,500 cells are seeded into each well of a 96-well flat bottom plate. The cells are incubated overnight to allow for cell attachment. Cells are then treated with Cisplatin in concentrations of 0-8 μg/mL alone or in combination with 1 μM of the HDAC inhibitor, M344. Forty eight hours following treatment, 42 μL of a 5 mg/mL MTT substrate solution in phosphate buffered saline (PBS) is added and incubated for up to 4 hrs at 37°C. The resulting violet formazan precipitate is solubilized by the addition of 82 μL of a 0.01 M HCl/10% SDS solution and plates are incubated overnight at 37°C. The plates are then analyzed on an MRX Microplate Reader at 570 nm to determine the optical density of the samples^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Jung M1, et al. Amide analogues of trichostatin A as inhibitors of histone deacetylase and inducers of terminal cell differentiation. J Med Chem. 1999 Nov 4;42(22):4669-79. [Content Brief]

[2]. Weberpals JI, et al. The effect of the histone deacetylase inhibitor M344 on BRCA1 expression in breast and ovarian cancer cells. Cancer Cell Int. 2011 Aug 19;11(1):29. [Content Brief]

M344 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2532 mL	16.2660 mL	32.5320 mL	81.3299 mL
	5 mM	0.6506 mL	3.2532 mL	6.5064 mL	16.2660 mL
	10 mM	0.3253 mL	1.6266 mL	3.2532 mL	8.1330 mL
	15 mM	0.2169 mL	1.0844 mL	2.1688 mL	5.4220 mL
	20 mM	0.1627 mL	0.8133 mL	1.6266 mL	4.0665 mL
	25 mM	0.1301 mL	0.6506 mL	1.3013 mL	3.2532 mL
	30 mM	0.1084 mL	0.5422 mL	1.0844 mL	2.7110 mL
	40 mM	0.0813 mL	0.4066 mL	0.8133 mL	2.0332 mL
	50 mM	0.0651 mL	0.3253 mL	0.6506 mL	1.6266 mL
	60 mM	0.0542 mL	0.2711 mL	0.5422 mL	1.3555 mL
	80 mM	0.0407 mL	0.2033 mL	0.4066 mL	1.0166 mL
	100 mM	0.0325 mL	0.1627 mL	0.3253 mL	0.8133 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

M344251456-60-7D 237 MS 344M 344M-344D237D-237MS344MS 344MS-344HDACHistone deacetylasesInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

M344 (Synonyms: D 237; MS 344)

Customer Review

Other Forms of M344:

MCE 顾客使用本产品发表的 2 篇科研文献