1. Autophagy
  2. Autophagy
  3. Schisandrin

Schisandrin  (Synonyms: 五味子醇甲; Schizandrin; Schizandrol; Schizandrol-A)

目录号: HY-N0691 纯度: 99.97%
COA 产品使用指南

Schisandrin (Schizandrin) 是一种从 Schisandra chinensis 的果实中分离出来的联苯环辛烯类木脂素。Schisandrin 具有抗氧化,保肝,抗癌和抗炎的活性。Schisandrin 还可以逆转大鼠的记忆障碍。

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Schisandrin Chemical Structure

Schisandrin Chemical Structure

CAS No. : 7432-28-2

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Other Forms of Schisandrin:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Schisandrin (Schizandrin), a dibenzocyclooctadiene lignan, is isolated from the fruit of Schisandra chinensis Baill. Schisandrin exhibits antioxidant, hepatoprotective, anti-cancer and anti-inflammatory activities. Schisandrin also can reverses memory impairment in rats[1][2][3].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
20.7 μM
Compound: Schizandrin
Antiproliferative activity against human A549 cells after 72 hrs by SRB assay
Antiproliferative activity against human A549 cells after 72 hrs by SRB assay
[PMID: 18063366]
A549 IC50
21.65 μM
Compound: 1
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
[PMID: 29501940]
A549 IC50
8.5 μM
Compound: 1
Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
[PMID: 32631552]
DU-145 IC50
13.09 μM
Compound: 1
Antiproliferative activity against human DU145 cells after 48 hrs by MTT assay
Antiproliferative activity against human DU145 cells after 48 hrs by MTT assay
[PMID: 29501940]
DU-145 IC50
5.38 μM
Compound: 1
Antiproliferative activity against human DU145 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Antiproliferative activity against human DU145 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
[PMID: 32631552]
H9 IC50
> 58 μM
Compound: schisandrin
Cytotoxicity against human H9 cells after 4 days
Cytotoxicity against human H9 cells after 4 days
[PMID: 17190445]
HCT-15 IC50
50.2 μM
Compound: Schizandrin
Antiproliferative activity against human HCT15 cells after 72 hrs by SRB assay
Antiproliferative activity against human HCT15 cells after 72 hrs by SRB assay
[PMID: 18063366]
HEK293 IC50
80.47 μM
Compound: 1
Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
[PMID: 32631552]
HEK293 IC50
81.74 μM
Compound: 1
Antiproliferative activity against HEK293 cells after 48 hrs by MTT assay
Antiproliferative activity against HEK293 cells after 48 hrs by MTT assay
[PMID: 29501940]
HeLa IC50
> 100 μM
Compound: 5
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
[PMID: 23237974]
HeLa IC50
28.56 μM
Compound: 1
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
[PMID: 29501940]
HeLa IC50
5.06 μM
Compound: 1
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
[PMID: 32631552]
K562 IC50
25 μM
Compound: Schizandrin
Antiproliferative activity against human K562 cells after 72 hrs by MTT assay
Antiproliferative activity against human K562 cells after 72 hrs by MTT assay
[PMID: 18063366]
Lewis lung carcinoma cell line IC50
> 100 μM
Compound: 5
Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
[PMID: 23237974]
MCF7 IC50
32.56 μM
Compound: 1
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
[PMID: 29501940]
MDA-MB-231 IC50
61.5 μM
Compound: Schizandrin
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay
[PMID: 18063366]
SK-HEP1 IC50
71.3 μM
Compound: Schizandrin
Antiproliferative activity against human SK-HEP1 cells after 72 hrs by SRB assay
Antiproliferative activity against human SK-HEP1 cells after 72 hrs by SRB assay
[PMID: 18063366]
SMMC-7721 IC50
> 100 μM
Compound: 5
Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay
Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay
[PMID: 23237974]
SNU-638 IC50
21.1 μM
Compound: Schizandrin
Antiproliferative activity against human SNU638 cells after 72 hrs by SRB assay
Antiproliferative activity against human SNU638 cells after 72 hrs by SRB assay
[PMID: 18063366]
T47D IC50
19.8 μM
Compound: Schizandrin
Antiproliferative activity against human T47D cells after 72 hrs by SRB assay
Antiproliferative activity against human T47D cells after 72 hrs by SRB assay
[PMID: 18063366]
U-87MG ATCC IC50
> 100 μM
Compound: 5
Cytotoxicity against human U87 cells after 72 hrs by MTT assay
Cytotoxicity against human U87 cells after 72 hrs by MTT assay
[PMID: 23237974]
体外研究
(In Vitro)

Schisandrin (10-100 μM; pretreated for 2 h) inhibits LPS-stimulated NO production, iNOS protein and mRNA expression in a dose-dependent manner in RAW 264.7 cells[1].
Schisandrin (25-100 μM; pretreated for 2 h) inhibits prostaglandin E2 production, COX-2 protein and mRNA expression in LPS-stimulated RAW 264.7 macrophages[1].
Schisandrin inhibits the SK-HEP-1, SNU-638, and T47D cells proliferation, with IC50s of 42.0, 53.1, and 40.0 μM, respectively[3].
Schisandrin (10-60 μM; 48 h) enhances Doxorubicin -induced apoptosis and selectively reverses MCF-7/DOX resistance[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: RAW 264.7 macrophages
Concentration: 12.5, 25, 50, 100 μM
Incubation Time: Pretreated for 2 h and then incubated with LPS (1 μg/mL) for 18 h
Result: Markedly decreased iNOS protein expression a dose-dependent manner.
Significantly inhibited COX-2 protein expression.
体内研究
(In Vivo)

Schisandrin (10-100 mg/kg; a single i.p.) inhibits acetic acid-induced peritoneal vascular permeability and reduces the carrageenan-induced paw edema of mice[1].
Schisandrin (1-10 mg/kg; a single p.o.) significantly reverses the Scopolamine-induced impairment of spatial memory and passive avoidance response, and enhances tremors induced by Oxotremorine in rats[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ICR mice (4-weeks) were treated with λ-carrageenan by intraperitoneal injection into the right hind paw[1]
Dosage: 100, 200 mg/kg
Administration: A single i.p.
Result: Inhibited paw edema by 33.43 % (100 mg/kg) and 57.38 % (200 mg/kg) at 3 h.
分子量

432.51

Formula

C24H32O7

CAS 号
性状

固体

颜色

White to light yellow

中文名称

五味子素;五味子醇甲

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验: 

DMSO 中的溶解度 : 1 mg/mL (2.31 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3121 mL 11.5604 mL 23.1209 mL
5 mM --- --- ---
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.28 mg/mL (0.65 mM); 澄清溶液

    此方案可获得 ≥ 0.28 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 2.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3121 mL 11.5604 mL 23.1209 mL 57.8021 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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