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  4. ALK-1 抗体 (YA2457)

ALK-1 抗体 (YA2457)

目录号: HY-P82712
COA 抗体使用指南 技术支持

ALK-1 Antibody (YA2457) 是无偶联基团的、靶向 ALK-1 的 IgG 抗体 (克隆号:YA2457),预测分子量为 56 kDa (实际观测:56 kDa)。ALK-1 Antibody (YA2457) 可用于人、 小鼠、 大鼠背景下的 WB 实验。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

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描述

ALK-1 Antibody (YA2457) is a non-conjugated IgG antibody, targeting ALK-1, with a predicted molecular weight of 56 kDa (observed band size: 56 kDa). ALK-1 Antibody (YA2457) can be used for WB experiment in human, mouse, rat background.

研究背景

ALK-1 是 TGF-β 家族配体 BMP9/GDF2 和 BMP10 的 I 型受体(type I receptor),在正常血管发育中起重要调控作用。当配体结合后,ALK-1 与两个 II 型受体(type II receptors)形成受体复合物,其中 II 型受体磷酸化并激活 ALK-1,使其自磷酸化后结合并激活 SMAD 转录调节因子(SMAD transcriptional regulators)。此外,ALK-1 可能结合激活素(activin)(By similarity)。

基因 ID
蛋白数据库
中文名
ALK-1 抗体 (YA2457)
同用名
ACVRL1; ACVRLK1; ALK1; Serine/threonine-protein kinase receptor R3; SKR3; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I
分子量

Predicted band size: 56 kDa; Observed band size: 56 kDa

纯度

Affinity Chromatography

偶联

Non-conjugated

修饰

Unmodified

RRID
研究领域

Cardiovascular

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1/500-1/1000

  • Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Mouse kidney(lane 2, 20 μg),Rat kidney(lane 3, 20 μg), and Mouse lung (lane 4, 20 μg) using ALK-1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
应用

WB

性状

液体

组分

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

A synthesized peptide derived from human ACVRL1

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
ALK-1 Antibody (YA2457)
目录号:
HY-P82712
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