2. Biology Dictionary
  3. Homogeneous Time Resolved Fluorescence

Homogeneous Time Resolved Fluorescence同质时间分辨荧光法

释义：

Homogeneous Time Resolved Fluorescence (HTRF) is a technique based on certain fluorescent dyes that exhibit unusually long fluorescence lifetimes. It measures reaction rates by eliminating background fluorescence interference and using non-radiative energy transfer or quenching between fluorescent rare earth metal chelates and different receptors. This method is widely used in drug screening, enzyme kinetics research, and molecular interaction detection, especially for experiments requiring high sensitivity and signal-to-noise ratios.

同质时间分辨荧光法是一种基于某些荧光染料具有异常长荧光寿命特性的方法。它通过消除背景荧光的影响，利用荧光稀土金属螯合物与不同受体之间的非辐射能量转移或猝灭来测量反应速率。这种方法广泛应用于药物筛选、酶动力学研究和分子相互作用检测，特别适合需要高灵敏度和高信噪比的实验。

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