2. Apoptosis TGF-beta/Smad Epigenetics Autophagy
  3. Apoptosis PKC Autophagy
  4. C8-Ceramide

C8-Ceramide  (Synonyms: N-辛酰基-D-神经鞘氨醇; N-Octanoyl-D-erythro-sphingosine)

目录号: HY-108391 纯度: ≥98.0%
COA 产品使用指南

摩尔计算器

C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) 是一种细胞渗透性的内源性神经酰胺类似物。C8-Ceramide 具有具有抗增殖特性，能作为化疗试剂。C8-Ceramide 能刺激树突状细胞促进 T 细胞对病毒感染的反应。C8-Ceramide 在体外能轻微地诱导 PKC 活化。

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C8-Ceramide Chemical Structure

C8-Ceramide Chemical Structure

CAS No. : 74713-59-0

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C8-Ceramide 相关抗体

Top Publications Citing Use of Products

查看 PKC 亚型特异性产品:

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PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKCι

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) is a cell-permeable analog of naturally occurring ceramides. C8-Ceramide has anti-proliferation properties and acts as a potent chemotherapeutic agent. C8-Ceramide stimulates dendritic cells to promote T cell responses upon virus infections. C8-Ceramide induces slight activation of protein kinase (PKC) in vitro[1][2][3][4].

IC50 & Target[2][4][5]

PKC

 

apoptosis

 

autophagy

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
MCF7 IC50
1.2 μM
Compound: C8-cer
Antiproliferative activity against tumor necrosis factor-resistant human MCF7 cells after 24 hrs by MTT assay
Antiproliferative activity against tumor necrosis factor-resistant human MCF7 cells after 24 hrs by MTT assay
[PMID: 19694470]
MCF7 IC50
4.2 μM
Compound: C8-cer
Inhibition of clonogenic survival of human MCF7 cells after 10 days by microscopy
Inhibition of clonogenic survival of human MCF7 cells after 10 days by microscopy
[PMID: 19694470]
MCF7 IC50
4.5 μM
Compound: C8-cer
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
[PMID: 19694470]
MCF7 IC50
8.6 μM
Compound: C8-cer
Inhibition of clonogenic survival of tumor necrosis factor-resistant human MCF7 cells after 10 days by microscopy
Inhibition of clonogenic survival of tumor necrosis factor-resistant human MCF7 cells after 10 days by microscopy
[PMID: 19694470]
MDA-MB-231 IC50
1.8 μM
Compound: C8-cer
Antiproliferative activity against endocrine-resistant human MDA-MB-231 cells after 24 hrs by MTT assay
Antiproliferative activity against endocrine-resistant human MDA-MB-231 cells after 24 hrs by MTT assay
[PMID: 19694470]
MDA-MB-231 IC50
3 μM
Compound: C8-cer
Inhibition of clonogenic survival of endocrine-resistant human MDA-MB-231 cells after 10 days by microscopy
Inhibition of clonogenic survival of endocrine-resistant human MDA-MB-231 cells after 10 days by microscopy
[PMID: 19694470]
体外研究
(In Vitro)

C8-ceramide (3 μM; 48 hours) irreversibly reduces tumor-cell proliferation and induces morphological changes[1].
C8-ceramide can induce necrosis-like cell death, but does not induce caspase-dependent cleavage of PARP (biochemical marker of apoptosis) in human cervical tumor cells[1].
C8-ceramide may increase the endogenous ROS level (10-30 µM; 24 hours) by regulating the switch of SOD1 and SOD2, causing the anti-proliferation (10-50 µM; 24 hours), and consequently triggering the apoptosis (10-50 µM; 48 hours) of NSCLC H1299 cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

C8-Ceramide 相关抗体:

Cell Viability Assay[1]

Cell Line: CALO cells, INBL cells, HeLa cells
Concentration: 3 μM
Incubation Time: 48 hours
Result: Markedly reduced the tumor cell number.

Cell Proliferation Assay[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 24 hours
Result: Decreased the rate of cellular proliferation in a dose-dependent manner, with an IC50 of 22.9 µM.

Cell Cycle Analysis[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 24 hours
Result: Caused the G1 arrest.

Apoptosis Analysis[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM
Incubation Time: 24 hours, 48 hours
Result: Increased the level of cleaved caspase-3.
体内研究
(In Vivo)

C8-ceramide (0.1 mg/kg; intranasal administration) induces more robust CD8+ and CD4+ T cell responses to viral infections in virus infected mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice, with lymphocytic choriomeningitis virus infected[3]
Dosage: 0.1 mg/kg
Administration: Intranasal administration
Result: Increased the CD8+ T cell response to influenza in the lungs.
分子量

425.69

Formula

C26H51NO3
CAS 号
性状

固体

颜色

White to off-white

中文名称

N-辛酰基-D-神经鞘氨醇
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : ≥ 100 mg/mL (234.91 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3491 mL 11.7456 mL 23.4913 mL
5 mM 0.4698 mL 2.3491 mL 4.6983 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: ≥98.0%

COA (255 KB) HNMR (284 KB) MS (205 KB)

产品使用指南 (1538 KB)
参考文献

C8-Ceramide 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3491 mL 11.7456 mL 23.4913 mL 58.7282 mL
5 mM 0.4698 mL 2.3491 mL 4.6983 mL 11.7456 mL
10 mM 0.2349 mL 1.1746 mL 2.3491 mL 5.8728 mL
15 mM 0.1566 mL 0.7830 mL 1.5661 mL 3.9152 mL
20 mM 0.1175 mL 0.5873 mL 1.1746 mL 2.9364 mL
25 mM 0.0940 mL 0.4698 mL 0.9397 mL 2.3491 mL
30 mM 0.0783 mL 0.3915 mL 0.7830 mL 1.9576 mL
40 mM 0.0587 mL 0.2936 mL 0.5873 mL 1.4682 mL
50 mM 0.0470 mL 0.2349 mL 0.4698 mL 1.1746 mL
60 mM 0.0392 mL 0.1958 mL 0.3915 mL 0.9788 mL
80 mM 0.0294 mL 0.1468 mL 0.2936 mL 0.7341 mL
100 mM 0.0235 mL 0.1175 mL 0.2349 mL 0.5873 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C8-Ceramide74713-59-0N-Octanoyl-D-erythro-sphingosineN-辛酰基-D-神经鞘氨醇ApoptosisPKCAutophagyProtein kinase Cceramidesanaloganti-proliferationcytotoxicchemotherapeuticagent,proteinkinaseactivationInhibitorinhibitorinhibit

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