ASAP2 - ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 Gene

中文名称：具有 SH3 结构域、锚蛋白重复序列和 PH 结构域 2 的 ArfGAP

种属: Homo sapiens

同用名: PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha

关于 ASAP2

功能概要

该基因编码一个多结构域蛋白，包含一个带有卷曲螺旋基序的 N 端 α 螺旋区域，然后是一个 pleckstrin 同源 (PH) 结构域、一个 Arf-GAP 结构域、一个锚蛋白同源区域、一个富含脯氨酸的区域，以及 C 端 Src 同源 3 (SH3) 结构域。该蛋白质定位于高尔基体和质膜，在那里它与蛋白酪氨酸激酶 2-β (Pyk2) 共定位。编码的蛋白质在体内与 Pyk2 形成稳定的复合物。这种相互作用似乎是通过其 SH3 结构域与 Pyk2 的 C 末端富含脯氨酸结构域的结合来介导的。编码的蛋白质是被激活的 Pyk2 磷酸化的酪氨酸。它在体外对 I 类和 II 类 ArfGAP 具有催化活性，并且可以在没有立即 GAP 活性的情况下结合 III 类 Arf ARF6。编码的蛋白质被认为作为 ARF GAP 起作用，当募集到高尔基体膜时控制 ARF 介导的囊泡出芽。此外，它还充当 Pyk2 和 Src 的底物和下游靶点，这是一种可能参与水泡转运调节的途径。已发现该基因编码不同异构体的多个转录变体。[RefSeq 提供，2008 年 9 月]

This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (Pyk2). The encoded protein forms a stable complex with Pyk2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of Pyk2. The encoded protein is tyrosine phosphorylated by activated Pyk2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for Pyk2 and Src, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]