1. Academic Validation
  2. Effects of the JAK2 inhibitor, AZ960, on Pim/BAD/BCL-xL survival signaling in the human JAK2 V617F cell line SET-2

Effects of the JAK2 inhibitor, AZ960, on Pim/BAD/BCL-xL survival signaling in the human JAK2 V617F cell line SET-2

  • J Biol Chem. 2008 Nov 21;283(47):32334-43. doi: 10.1074/jbc.M803813200.
Joseph M Gozgit 1 Geraldine Bebernitz Pankaj Patil Minwei Ye Julie Parmentier Jiaquan Wu Nancy Su Tao Wang Stephanos Ioannidis Audrey Davies Dennis Huszar Michael Zinda
Affiliations

Affiliation

  • 1 Cancer Biosciences, AstraZeneca R&D Boston, Waltham, Massachusetts 02451, USA.
Abstract

The Janus-associated kinase 2 (JAK2) V617F mutation is believed to play a critical role in the pathogenesis of polycythemia vera, essential thrombocythemia, and idiopathic myelofibrosis. We have characterized a novel small molecule JAK2 Inhibitor, AZ960, and used it as a tool to investigate the consequences of JAK2 V617F inhibition in the SET-2 cell line. AZ960 inhibits JAK2 kinase with a K(i) of 0.00045 microm in vitro and treatment of TEL-JAK2 driven Ba/F3 cells with AZ960 blocked STAT5 phosphorylation and potently inhibited cell proliferation (GI(50)=0.025 microm). AZ960 demonstrated selectivity for TEL-JAK2-driven STAT5 phosphorylation and cell proliferation when compared with cell lines driven by similar fusions of the other JAK kinase family members. In the SET-2 human megakaryoblastic cell line, heterozygous for the JAK2 V617F allele, inhibition of JAK2 resulted in decreased STAT3/5 phosphorylation and inhibition of cell proliferation (GI(50)=0.033 microm) predominately through the induction of mitochondrial-mediated Apoptosis. We provide evidence that JAK2 inhibition induces Apoptosis by direct and indirect regulation of the anti-apoptotic protein Bcl-xL. Inhibition of JAK2 blocked Bcl-xL mRNA expression resulting in a reduction of Bcl-xL protein levels. Additionally, inhibition of JAK2 resulted in decreased PIM1 and PIM2 mRNA expression. Decreased PIM1 mRNA corresponded with a decrease in PIM1 protein levels and inhibition of BAD phosphorylation at Ser(112). Finally, small interfering RNA-mediated suppression of Bcl-xL resulted in apoptotic cell death similar to the phenotype observed following JAK2 inhibition. These results suggest a model in which JAK2 promotes cell survival by signaling through the Pim/BAD/Bcl-xL pathway.

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