2. Academic Validation
  3. Nek11 regulates asymmetric cell division during mouse oocyte meiotic maturation

Nek11 regulates asymmetric cell division during mouse oocyte meiotic maturation

  • Biochem Biophys Res Commun. 2016 Jun 10;474(4):667-672. doi: 10.1016/j.bbrc.2016.05.002.
Lei Guo 1,  Zhen-Bo Wang 1,  Hong-Hui Wang 1,  Teng Zhang 1,  Shu-Tao Qi 1,  Ying-Chun Ouyang 1,  Yi Hou 1,  Qing-Yuan Sun 2
Affiliations

Affiliations

  • 1 State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
  • 2 State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China. Electronic address: sunqy@ioz.ac.cn.
PMID: 27150633 DOI: 10.1016/j.bbrc.2016.05.002
Abstract

Nek11, a member of the never in mitosis gene A (NIMA) family, is activated in somatic cells associated with G1/S or G2/M arrest. However, its function in meiosis is unknown. In this research, the expression, localization and functions of NEK11 in the mouse oocyte meiotic maturation were examined. Western blotting indicated that NEK11S was the major NEK11 protein in mouse oocyte. MYC-tagged Nek11 mRNA microinjection and immunofluorescent staining showed that NEK11 was localized to the meiotic spindles at MI and MII stage. Knockdown of Nek11 by microinjection of siRNA did not affect germinal vesicle breakdown (GVBD) and the first polar body extrusion, but caused formation of 2-cell-like eggs. These results demonstrate that Nek11 regulates asymmetric cell division during oocyte meiotic maturation.

Keywords

Meiosis; Nek11; Oocyte maturation; Symmetric division.

Figures
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z