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  3. Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants

Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants

  • Chem Commun (Camb). 2017 Nov 14;53(91):12309-12312. doi: 10.1039/c7cc06661a.
Michiko Kimoto 1,  Adam J Meyer,  Ichiro Hirao,  Andrew D Ellington
Affiliations

Affiliation

  • 1 Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #09-01, Singapore 138669, Singapore. ichiro@ibn.a-star.edu.sg.
PMID: 29094732 DOI: 10.1039/c7cc06661a
Abstract

Thermostable T7 RNA polymerase variants were explored for genetic alphabet expansion transcription involving the unnatural Ds-Pa pair. One variant exhibited high incorporation efficiencies of functionally modified Pa substrates and enabled the simultaneous incorporation of 2'-fluoro-nucleoside triphosphates of pyrimidines into transcripts, allowing the generation of novel, highly functional RNA molecules.

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