2. Academic Validation
  3. Extracellular Guanosine 5'-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine

Extracellular Guanosine 5'-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine

  • Front Pharmacol. 2018 Mar 16:9:152. doi: 10.3389/fphar.2018.00152.
Tiziana Pietrangelo 1,  Ester S Di Filippo 1,  Marcello Locatelli 2,  Francesco Piacenza 3,  Marco Farina 4,  Eleonora Pavoni 4,  Andrea Di Donato 5,  Denise Innosa 6,  Mauro Provinciali 3,  Stefania Fulle 1
Affiliations

Affiliations

  • 1 Dipartimento Neuroscienze Imaging and Scienze Cliniche, Università degli Studi "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
  • 2 Dipartimento di Farmacia, Università degli Studi "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
  • 3 IRCCS-Istituto Nazionale di Riposo e Cura per Anziani, Polo Scientifico e Tecnologico, Centro di Tecnologie Avanzate nell'Invecchiamento, Ancona, Italy.
  • 4 Dipartimento di Ingegneria dell'Informazione, Università Politecnica delle Marche, Ancona, Italy.
  • 5 Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, United States.
  • 6 Facoltà di Bioscienze e Tecnologie Agro-Alimentari e Ambientali, Università di Teramo, Teramo, Italy.
PMID: 29615899 DOI: 10.3389/fphar.2018.00152
Abstract

The extracellular guanosine 5'-triphosphate, GTP, has been demonstrated to be an enhancer of myogenic cell differentiation in a murine cell line, not yet in human muscle cells. Our hypothesis was that GTP could influence also human skeletal muscle regeneration, specifically in the first phases. We tested GTP stimulus on human muscle precursor cells established in culture by human satellite cells derived from Vastus Lateralis of three young male. Our data show that extracellular GTP (a) up-regulated miRNA (specifically miR133a and miR133b) and myogenic regulator factor and (b) induces human myogenic precursor cells to release exosomes stuffed with guanosine based molecules (mainly guanosine) in the extracellular milieu. We think that probably these exosomes could be addressed to influence by means of their content (mainly guanosine) in paracrine or autocrine manner the surrounding cells and/or at distance other muscles or tissues.

Keywords

exosomes; guanosine; guanosine 5′-triphosphate; myomiRNA; satellite cells; skeletal muscle regeneration.

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