2. Academic Validation
  3. Curcumol Exerts Anticancer Effect in Cholangiocarcinoma Cells via Down-Regulating CDKL3

Curcumol Exerts Anticancer Effect in Cholangiocarcinoma Cells via Down-Regulating CDKL3

  • Front Physiol. 2018 Mar 20;9:234. doi: 10.3389/fphys.2018.00234.
Jinduo Zhang 1 2 3 4 5,  Gang Su 1 3,  Zengwei Tang 1 2 4 5,  Li Wang 3 6,  Wenkang Fu 1 2 4 5,  Sheng Zhao 1 3,  Yongjiang Ba 1 2 4 5,  Bing Bai 1 2 4 5,  Ping Yue 1 2 4 5,  Yanyan Lin 1 2 4 5,  Zhongtian Bai 1 4 5 7,  Jinjing Hu 1 4 5,  Wenbo Meng 1 2 3 4 5,  Liang Qiao 8,  Xun Li 1 4 5 7,  Xiaodong Xie 1 3
Affiliations

Affiliations

  • 1 The First Clinical Medical College, Lanzhou University, Lanzhou, China.
  • 2 Special Minimally Invasive Surgery, The First Hospital of Lanzhou University, Lanzhou, China.
  • 3 School of Basic Medical Sciences, Institute of Genetics, Lanzhou University, Lanzhou, China.
  • 4 Gansu Province Institute of Hepatopancreatobiliary, Lanzhou, China.
  • 5 Gansu Province Key Laboratory Biotherapy and Regenerative Medicine, Lanzhou, China.
  • 6 School of Stomatology, Lanzhou University, Lanzhou, China.
  • 7 The Second Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou, China.
  • 8 Storr Liver Centre, Westmead Institute for Medical Research, University of Sydney, Westmead, NSW, Australia.
PMID: 29615928 DOI: 10.3389/fphys.2018.00234
Abstract

Curcumol is the major component extracted from root of Rhizoma Curcumae. Recent studies have shown that curcumol exerts therapeutic effects against multiple conditions, particularly cancers. However, the therapeutic role and mechanism of curcumol against cholangiocarcinoma cells are still unclear. In our current research, we tested the effect of curcumol in cholangiocarcinoma cells, and using two-dimensional electrophoresis, proteomics and bioinformatics, we identified cyclin-dependent kinase like 3 (CDKL3) as a potential target for curcumol. We have demonstrated that curcumol can evidently suppress growth and migration of cholangiocarcinoma cells. Furthermore, curcumol could significantly block the cell cycle progression of the cholangiocarcinoma cells. These effects could be largely attributed to the inhibition of CDKL3 by curcumol. Further studies have recapitulated the oncogenic role of CDKL3 in that knockdown of CDKL3 by lentiviral mediated transfection of shRNA against CDKL3 also led to a significant inhibition on cell proliferation, migration, invasion, and cell cycle progression. Given the high level of CDKL3 expression in human cholangiocarcinoma tissues and cell lines, we speculated that CDKL3 may constitute a potential biological target for curcumol in cholangiocarcinoma.

Keywords

CDKL3; cell cycle; cholangiocarcinoma; curcumol; proteomics.

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