2. Academic Validation
  3. NLRP3 inflammasome-mediated pyroptosis contributes to the pathogenesis of non-ischemic dilated cardiomyopathy

NLRP3 inflammasome-mediated pyroptosis contributes to the pathogenesis of non-ischemic dilated cardiomyopathy

  • Redox Biol. 2020 Jul;34:101523. doi: 10.1016/j.redox.2020.101523.
Cheng Zeng 1,  Fengqi Duan 1,  Jia Hu 1,  Bin Luo 2,  Binlong Huang 3,  Xiaoying Lou 4,  Xiuting Sun 5,  Hongyu Li 6,  Xuanhong Zhang 1,  Shengli Yin 7,  Hongmei Tan 8
Affiliations

Affiliations

  • 1 Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
  • 2 Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
  • 3 Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
  • 4 Department of Pathology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510655, China.
  • 5 Department of Cardiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
  • 6 Laboratory Animal Center, Sun Yat-sen University, Guangzhou, 510080, China.
  • 7 Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China. Electronic address: yinshl@mail.sysu.edu.cn.
  • 8 Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China; Guangdong Engineering & Technology Research Center for Disease-Model Animals, Sun Yat-sen University, Guangzhou, 510080, China. Electronic address: tanhm@mail.sysu.edu.cn.
PMID: 32273259 DOI: 10.1016/j.redox.2020.101523
Abstract

Dilated cardiomyopathy (DCM) is one of the most common causes of heart failure, and the underlying mechanism remains largely elusive. Here we investigated whether NLRP3 inflammasome-mediated Pyroptosis contributes to non-ischemic DCM and dissected the underlying mechanism. We found that hyper activated NLRP3 inflammasome with pyroptotic cell death of cardiomyocytes were presented in the myocardial tissues of DCM patients, which were negatively correlated with cardiac function. Doxorubicin (Dox)-induced DCM characterization disclosed that NLRP3 inflammasome activation and Pyroptosis occurred in Dox-treated heart tissues, but were very marginal in either NLRP3-/- or Caspase-1-/- mice. Mechanistically, Dox enhanced expressions of NOX1 and NOX4 and induced mitochondrial fission through dynamin-related protein 1 (Drp1) activation, leading to NLRP3 inflammasome-mediated Pyroptosis in cardiomyocytes via caspase-1-dependent manner. Conversely, both inhibitions of NOX1 and NOX4 and Drp1 suppressed Dox-induced NLPR3 inflammasome activation and Pyroptosis. The alterations of NOX1 and NOX4 expression, Drp1 phosphorylation and mitochondrial fission were validated in DCM patients and mice. Importantly, Dox-induced Drp1-mediated mitochondrial fission and the consequent NLRP3 inflammasome activation and Pyroptosis were reversed by NOX1 and NOX4 inhibition in mice. This study demonstrates for the first time that cardiomyocyte Pyroptosis triggered by NLRP3 inflammasome activation via Caspase-1 causally contributes to myocardial dysfunction progression and DCM pathogenesis.

Keywords

Dilated cardiomyopathy; Heart failure; Mitochondrial fission; NLRP3 inflammasome; NOX; Pyroptosis.

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