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  3. Mechanical force induces macrophage-derived exosomal UCHL3 promoting bone marrow mesenchymal stem cell osteogenesis by targeting SMAD1

Mechanical force induces macrophage-derived exosomal UCHL3 promoting bone marrow mesenchymal stem cell osteogenesis by targeting SMAD1

  • J Nanobiotechnology. 2023 Mar 14;21(1):88. doi: 10.1186/s12951-023-01836-z.
Panjun Pu # 1 2 3 4,  Shengnan Wu # 1 2 3,  Kejia Zhang 1 2 3,  Hao Xu 1 2 3,  Jiani Guan 1 2 3,  Zhichun Jin 1 2 3,  Wen Sun 2,  Hanwen Zhang 5 6,  Bin Yan 7 8 9
Affiliations

Affiliations

  • 1 Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, 210000, China.
  • 2 Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 210000, China.
  • 3 Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, 210000, China.
  • 4 Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, 710049, China.
  • 5 School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 210000, China. hanwenzhang@njmu.edu.cn.
  • 6 Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, 210000, China. hanwenzhang@njmu.edu.cn.
  • 7 Department of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, 210000, China. byan@njmu.edu.cn.
  • 8 Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 210000, China. byan@njmu.edu.cn.
  • 9 Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, 210000, China. byan@njmu.edu.cn.
  • # Contributed equally.
PMID: 36915132 DOI: 10.1186/s12951-023-01836-z
Abstract

Background: Orthodontic tooth movement (OTM), a process of alveolar bone remodelling, is induced by mechanical force and regulated by local inflammation. Bone marrow-derived mesenchymal stem cells (BMSCs) play a fundamental role in osteogenesis during OTM. Macrophages are mechanosensitive cells that can regulate local inflammatory microenvironment and promote BMSCs osteogenesis by secreting diverse mediators. However, whether and how mechanical force regulates osteogenesis during OTM via macrophage-derived exosomes remains elusive.

Results: Mechanical stimulation (MS) promoted bone marrow-derived macrophage (BMDM)-mediated BMSCs osteogenesis. Importantly, when exosomes from mechanically stimulated BMDMs (MS-BMDM-EXOs) were blocked, the pro-osteogenic effect was suppressed. Additionally, compared with exosomes derived from BMDMs (BMDM-EXOs), MS-BMDM-EXOs exhibited a stronger ability to enhance BMSCs osteogenesis. At in vivo, mechanical force-induced alveolar bone formation was impaired during OTM when exosomes were blocked, and MS-BMDM-EXOs were more effective in promoting alveolar bone formation than BMDM-EXOs. Further proteomic analysis revealed that ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCHL3) was enriched in MS-BMDM-EXOs compared with BMDM-EXOs. We went on to show that BMSCs osteogenesis and mechanical force-induced bone formation were impaired when UCHL3 was inhibited. Furthermore, mothers against decapentaplegic homologue 1 (SMAD1) was identified as the target protein of UCHL3. At the mechanistic level, we showed that SMAD1 interacted with UCHL3 in BMSCs and was downregulated when UCHL3 was suppressed. Consistently, overexpression of SMAD1 rescued the adverse effect of inhibiting UCHL3 on BMSCs osteogenesis.

Conclusions: This study suggests that mechanical force-induced macrophage-derived exosomal UCHL3 promotes BMSCs osteogenesis by targeting SMAD1, thereby promoting alveolar bone formation during OTM.

Keywords

Exosomes; Macrophages; Mechanical force; Orthodontic tooth movement; Osteogenesis; SMAD1; UCHL3.

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