1. Anti-infection
  2. Bacterial
  3. Methyl caffeate

Methyl caffeate  (Synonyms: 咖啡酸甲酯)

目录号: HY-N6005 纯度: 99.89%
COA 产品使用指南

Methyl caffeate 是一种抗微生物剂,具有中等的抗菌和良好的抗分枝杆菌活性。Methyl caffeate 还表现出 α-葡萄糖苷酶抑制活性,氧化应激抑制活性,抗血小板活性,子宫颈癌的抗增殖活性以及肺和白细胞的抗癌活性。

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Methyl caffeate Chemical Structure

Methyl caffeate Chemical Structure

CAS No. : 3843-74-1

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3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥397
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25 mg ¥361
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50 mg ¥500
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100 mg ¥800
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200 mg   询价  
500 mg   询价  

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Customer Review

Other Forms of Methyl caffeate:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Methyl caffeate, an antimicrobial agent, shows moderate antimicrobial and prominent antimycobacterial activities. Methyl caffeate also exhibits α-glucosidase inhibition activity, oxidative stress inhibiting activity, anti-platelet activity, antiproliferative activity in cervix adenocarcinoma and anticancer activity in lung and leukmia cell lines[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
7.54 μM
Compound: 2a
Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay
Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay
[PMID: 22954735]
BeWo IC50
17.27 μM
Compound: I-1
Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay
[PMID: 25160837]
Calu-1 IC50
5.5 μM
Compound: 2a
Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay
Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay
[PMID: 22954735]
HeLa IC50
3.77 μM
Compound: 2a
Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay
Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay
[PMID: 22954735]
HeLa IC50
52.53 μM
Compound: I-1
Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay
[PMID: 25160837]
HepG2 IC50
81.55 μM
Compound: I-1
Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay
[PMID: 25160837]
HL-60 IC50
12.32 μM
Compound: I-1
Antitumor activity against human HL60 cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human HL60 cells assessed as inhibition of cell growth by MTT assay
[PMID: 25160837]
HL-60 IC50
7.5 μM
Compound: 4
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
[PMID: 15387667]
HOP-62 IC50
3.86 μM
Compound: 2a
Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay
Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay
[PMID: 22954735]
HT-1080 IC50
11.1 μM
Compound: 4
Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
[PMID: 15387667]
KB IC50
> 200 μM
Compound: 1d
Antitumor activity against KB cells by MTT assay
Antitumor activity against KB cells by MTT assay
[PMID: 17387015]
LNCaP IC50
> 100 μM
Compound: 3
Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay
Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay
[PMID: 24080105]
LoVo IC50
> 40 μM
Compound: 4
Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay
Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay
[PMID: 15387667]
LoVo IC50
5.8 μM
Compound: 4
Cytotoxicity against human LoVo cells after 72 hrs by MTT assay
Cytotoxicity against human LoVo cells after 72 hrs by MTT assay
[PMID: 15387667]
LOX IMVI IC50
21.8 μM
Compound: 2a
Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay
Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay
[PMID: 22954735]
M14 IC50
8.86 μM
Compound: 2a
Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay
Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay
[PMID: 22954735]
NCI-H1299 IC50
3.53 μM
Compound: 2a
Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay
[PMID: 22954735]
NCI-H157 IC50
6.25 μM
Compound: 2a
Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay
[PMID: 22954735]
NCI-H1792 IC50
6.09 μM
Compound: 2a
Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay
[PMID: 22954735]
NCI-H460 IC50
14.8 μM
Compound: 2a
Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay
Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay
[PMID: 22954735]
Oocyte IC50
388 μM
Compound: Caf-COOMe, methyl caffeate
Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique
Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique
[PMID: 23682762]
RAW264.7 IC50
> 0.3 mM
Compound: 31
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs
[PMID: 18986199]
RAW264.7 EC50
3.199 μM
Compound: 2, methyl caffeate
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay
[PMID: 18667320]
RAW264.7 IC50
3.9 μM
Compound: 29
Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA
[PMID: 31747281]
RAW264.7 EC50
367.5 μM
Compound: 2, methyl caffeate
Cytotoxicity against mouse RAW264.7 cells assessed as cell survival after 24 hrs by MTT assay
Cytotoxicity against mouse RAW264.7 cells assessed as cell survival after 24 hrs by MTT assay
[PMID: 18667320]
SGC-7901 IC50
> 100 μM
Compound: I-1
Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay
[PMID: 25160837]
SiHa IC50
60.26 μM
Compound: I-1
Antitumor activity against human SiHa cells assessed as inhibition of cell growth by MTT assay
Antitumor activity against human SiHa cells assessed as inhibition of cell growth by MTT assay
[PMID: 25160837]
分子量

194.18

Formula

C10H10O4

CAS 号
性状

固体

颜色

White to off-white

中文名称

咖啡酸甲酯

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验: 

DMSO 中的溶解度 : 33.33 mg/mL (171.64 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.1499 mL 25.7493 mL 51.4986 mL
5 mM 1.0300 mL 5.1499 mL 10.2997 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (12.87 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (12.87 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

    2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.89%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.1499 mL 25.7493 mL 51.4986 mL 128.7465 mL
5 mM 1.0300 mL 5.1499 mL 10.2997 mL 25.7493 mL
10 mM 0.5150 mL 2.5749 mL 5.1499 mL 12.8747 mL
15 mM 0.3433 mL 1.7166 mL 3.4332 mL 8.5831 mL
20 mM 0.2575 mL 1.2875 mL 2.5749 mL 6.4373 mL
25 mM 0.2060 mL 1.0300 mL 2.0599 mL 5.1499 mL
30 mM 0.1717 mL 0.8583 mL 1.7166 mL 4.2916 mL
40 mM 0.1287 mL 0.6437 mL 1.2875 mL 3.2187 mL
50 mM 0.1030 mL 0.5150 mL 1.0300 mL 2.5749 mL
60 mM 0.0858 mL 0.4292 mL 0.8583 mL 2.1458 mL
80 mM 0.0644 mL 0.3219 mL 0.6437 mL 1.6093 mL
100 mM 0.0515 mL 0.2575 mL 0.5150 mL 1.2875 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Methyl caffeate
目录号:
HY-N6005
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