Methyl p-coumarate (Synonyms: 4-羟基肉桂酸甲酯; Methyl 4-hydroxycinnamate)

目录号: HY-N1434 纯度: 99.80%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Methyl p-coumarate (Methyl 4-hydroxycinnamate)，一种可从 Trixis michuacana var longifolia 的花中分离出来的具有口服活性的对香豆酸 (pCA) 的酯化衍生物。Methyl p-coumarate 可抑制 B16 小鼠黑素瘤细胞中黑色素的形成。Methyl p-coumarate 对链格孢菌和其他病原体也具有很强的体外抑制作用。

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Methyl p-coumarate Chemical Structure

CAS No. : 3943-97-3

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥110	In-stock
5 g	￥100	In-stock
10 g	￥160	In-stock
50 g		询价

or 大包装询价

* Please select Quantity before adding items.

Other Forms of Methyl p-coumarate:

(E)-Methyl 4-coumarate In-stock

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生物活性
纯度 & 产品资料
参考文献

生物活性

Methyl p-coumarate (Methyl 4-hydroxycinnamate), an orally active esterified derivative of p-Coumaric acid (pCA), can be isolated from the flower of Trixis michuacana var longifolia. Methyl p-coumarate could inhibit the melanin formation in B16 mouse melanoma cells. Methyl p-coumarate also has strong in vitro inhibitory effect on A. alternata and other pathogens^[1]^[2].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	118.8 μM Compound: 4	Antiadipogenic activity in mouse 3T3L1 cells assessed as differentiation of preadipocytes to adipocytes after 8 days by spectrophotometry Antiadipogenic activity in mouse 3T3L1 cells assessed as differentiation of preadipocytes to adipocytes after 8 days by spectrophotometry	[PMID: 23628332]
A549	IC₅₀	322.92 μM Compound: pcm	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30615450]
HaCaT	IC₅₀	311.22 μM Compound: pcm	Cytotoxicity against human HaCaT cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HaCaT cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30615450]
HeLa	IC₅₀	> 150 μM Compound: pcm	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30615450]
HT-1080	ED₅₀	> 100 μM Compound: 16	Antiproliferative activity against human HT1080 cells by MTT assay Antiproliferative activity against human HT1080 cells by MTT assay	[PMID: 11277741]
KB	IC₅₀	123.1 μM Compound: 1b	Antitumor activity against KB cells by MTT assay Antitumor activity against KB cells by MTT assay	[PMID: 17387015]
MCF7	IC₅₀	> 400 μM Compound: pcm	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30615450]
NCI-H460	IC₅₀	120.01 μM Compound: pcm	Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30615450]
NCI-H661	IC₅₀	289.21 μM Compound: pcm	Cytotoxicity against human NCI-H661 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human NCI-H661 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30615450]
RAW264.7	GI₅₀	609 μM Compound: 2a	Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay	10.1039/C3MD00251A
SiHa	IC₅₀	> 150 μM Compound: pcm	Cytotoxicity against human SiHa cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human SiHa cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30615450]

体外研究
(In Vitro)

Methyl p-coumarate (10 μM) 抑制 B16 小鼠黑色素瘤细胞中黑色素的形成^[1]。
Methyl p-coumarate (200 μg/mL) 显示出针对 A. alternata 的抗真菌活性，并抑制枣果链格孢属 (Alternaria) 腐烂^[2]。
Methyl p-coumarate (0-100 μM，1 小时) 通过抑制 IL-6 的分泌，对 Phorbol 12-myristate 13-acetate (HY-18739) (10 nM，18 小时) 刺激的 A549 细胞具有抗炎活性、IL-8、MCP-1 和 ICAM-1^[3]。
Methyl p-coumarate (0-100 μM，1 小时) 抑制 Phorbol 12-myristate 13-acetate (HY-18739) (10 nM，18 小时) 刺激的 A549 细胞中 NF-κB/AP-1 的激活^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	Phorbol 12-myristate 13-acetate (HY-18739) (10 nM, 1 h)-stimulated A549 cells
Concentration:	0-100 μM
Incubation Time:	1 h
Result:	Inhibited PMA-stimulated phosphorylation of IκBα and p65, as well as c-Jun/c-Fos phosphorylation and c-Jun/c-Fos nuclear translocation.

体内研究
(In Vivo)

Methyl p-coumarate (5 mg/kg，口服) 可抑制 Ovalbumins (HY-W250978) 诱导的过敏性哮喘小鼠的气道炎症^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Ovalbumins (HY-W250978)-induced mice with allergic asthma^[3]
Dosage:	5 mg/kg
Administration:	p.o.
Result:	Inhibited the secretion of Th2 cytokines, MCP-1, and IgE. Inhibited eosinophil/macrophage influx. Inhibited immune cell influx and mucus secretion.

分子量

178.18

Formula

C₁₀H₁₀O₃

CAS 号

3943-97-3

性状

固体

颜色

Off-white to light yellow

中文名称

4-羟基肉桂酸甲酯；对羟基肉桂酸甲酯

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

细胞实验：

DMSO 中的溶解度 : 100 mg/mL (561.23 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.6123 mL	28.0615 mL	56.1230 mL
5 mM	1.1225 mL	5.6123 mL	11.2246 mL
10 mM	0.5612 mL	2.8062 mL	5.6123 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (14.03 mM); 悬浊液; 超声助溶

此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.03 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.03 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.80%

选择批次：

Data Sheet (626 KB) SDS (393 KB)

COA (283 KB) HNMR (130 KB) RP-HPLC (227 KB) LCMS (97 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Kubo I, et, al. Methyl p-coumarate, a melanin formation inhibitor in B16 mouse melanoma cells. Bioorg Med Chem. 2004 Oct 15;12(20):5349-54. [Content Brief]

[2]. Yuan S, et, al. Defense Responses, Induced by p-Coumaric Acid and Methyl p-Coumarate, of Jujube ( Ziziphus jujuba Mill.) Fruit against Black Spot Rot Caused by Alternaria alternate. J Agric Food Chem. 2019 Mar 13;67(10):2801-2810. [Content Brief]

[3]. Park JW, et al. Methyl P-Coumarate Ameliorates the Inflammatory Response in Activated-Airway Epithelial Cells and Mice with Allergic Asthma. Int J Mol Sci. 2022 Nov 28;23(23):14909. [Content Brief]

Methyl p-coumarate 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6123 mL	28.0615 mL	56.1230 mL	140.3076 mL
	5 mM	1.1225 mL	5.6123 mL	11.2246 mL	28.0615 mL
	10 mM	0.5612 mL	2.8062 mL	5.6123 mL	14.0308 mL
	15 mM	0.3742 mL	1.8708 mL	3.7415 mL	9.3538 mL
	20 mM	0.2806 mL	1.4031 mL	2.8062 mL	7.0154 mL
	25 mM	0.2245 mL	1.1225 mL	2.2449 mL	5.6123 mL
	30 mM	0.1871 mL	0.9354 mL	1.8708 mL	4.6769 mL
	40 mM	0.1403 mL	0.7015 mL	1.4031 mL	3.5077 mL
	50 mM	0.1122 mL	0.5612 mL	1.1225 mL	2.8062 mL
	60 mM	0.0935 mL	0.4677 mL	0.9354 mL	2.3385 mL
	80 mM	0.0702 mL	0.3508 mL	0.7015 mL	1.7538 mL
	100 mM	0.0561 mL	0.2806 mL	0.5612 mL	1.4031 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methyl p-coumarate3943-97-3Methyl 4-hydroxycinnamate4-羟基肉桂酸甲酯对羟基肉桂酸甲酯FungalTrixismichuacanalongifoliamelaninformationB16melanomaA. alternatapathogensInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

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上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Methyl p-coumarate (Synonyms: 4-羟基肉桂酸甲酯; Methyl 4-hydroxycinnamate)

Other Forms of Methyl p-coumarate: