2. Cytoskeleton
  3. Collagen
  4. Neutral protease I

Neutral protease I  (Synonyms: Dispase I)

目录号: HY-131577A
COA 产品使用指南 技术支持

Neutral protease I (Dispase I) 是一种快速、有效、温和的中性蛋白酶，可以将完整的表皮层与真皮层分开。Neutral protease I 还可以将培养物中的完整上皮细胞层与下层分离。Neutral protease I 可在消化基底膜区域的同时保留上皮细胞的活力。Neutral protease I 还可用于防止悬浮培养物中的细胞结块。Neutral protease I 可水解纤连蛋白和IV型胶原蛋白，但不能水解层粘连蛋白、V型胶原蛋白、血清白蛋白或转铁蛋白。

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Neutral protease I

Neutral protease I Chemical Structure

CAS No. : 42613-33-2

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1 mg ￥3500
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Other Forms of Neutral protease I:

Neutral protease I 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Neutral protease I (Dispase I) is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. Neutral protease I can also separate intact epithelial sheets in culture from the substratum. Neutral protease I preserves the viability of the epithelial cells while cleaving the basement membrane zone region. Neutral protease I can also be used to prevent clumping in suspension cultures. Neutral protease I cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin[1].

CAS 号
性状

固体

颜色

White to off-white

酶活

≥10 U/mg soild
单位定义

One unit is defined as the amount of enzyme that can hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料

COA (275 KB)

产品使用指南 (1538 KB)
参考文献

Neutral protease I 相关分类

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Neutral protease I42613-33-2Dispase ICollagenEDTA、EGTA、Hg2+Inhibitorinhibitorinhibit

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