2. Apoptosis
  3. Apoptosis
  4. Physalin F

Physalin F  (Synonyms: 酸浆苦味素F)

目录号: HY-N7696 纯度: 98.17%
COA 产品使用指南

摩尔计算器

Physalin F 是一种具有强烈抗炎和免疫调节作用的分泌型甾体。Physalin F 诱导人外周血单个核细胞凋亡，降低人 T 淋巴细胞 1 型病毒 (HTLV-1) 感染后的自发增殖和细胞因子的产生。

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Physalin F Chemical Structure

Physalin F Chemical Structure

CAS No. : 57423-71-9

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规格 价格 是否有货 数量
1 mg ￥550
In-stock
5 mg ￥1100
In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Customer Review

Physalin F 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Physalin F is a secosteroid with potent anti-inflammatory and immunomodulatory activities. Physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by Human T-lymphotropic virus type 1 (HTLV-1) infection[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
> 50 μM
Compound: 22
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
[PMID: 35608269]
HepG2 IC50
> 50 μM
Compound: 22
Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
[PMID: 35608269]
HT-1080 IC50
2.41 μM
Compound: Physalin F
Antiproliferative activity against human HT-1080 cells assessed as reduction in cell proliferation measured after 48 hrs by CCK8 assay
Antiproliferative activity against human HT-1080 cells assessed as reduction in cell proliferation measured after 48 hrs by CCK8 assay
[PMID: 33735687]
L02 IC50
> 50 μM
Compound: 22
Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
[PMID: 35608269]
MCF7 IC50
> 50 μM
Compound: 22
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
[PMID: 35608269]
MCF7 IC50
0.6 μM
Compound: 8
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
[PMID: 33586438]
NCI-H460 IC50
1 μM
Compound: 8
Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
[PMID: 33586438]
PC-3 IC50
1 μM
Compound: 8
Cytotoxicity against human PC-3 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human PC-3 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
[PMID: 33586438]
RAW264.7 IC50
> 50 μM
Compound: 22
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
[PMID: 35608269]
RAW264.7 IC50
7.9 μM
Compound: 22
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay
[PMID: 35608269]
SF-268 IC50
0.5 μM
Compound: 8
Cytotoxicity against human SF-268 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human SF-268 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
[PMID: 33586438]
SGC-7901 IC50
> 50 μM
Compound: 22
Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
[PMID: 35608269]
WI-38 IC50
3.5 μM
Compound: 8
Cytotoxicity against human WI-38 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human WI-38 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
[PMID: 33586438]
体外研究
(In Vitro)

Physalin F is a seco-steroid from Physalis angulate L.[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Physalin F 相关抗体:
分子量

526.53

Formula

C28H30O10
CAS 号
性状

固体

颜色

White to off-white

中文名称

酸浆苦味素F
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (189.92 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8992 mL 9.4961 mL 18.9923 mL
5 mM 0.3798 mL 1.8992 mL 3.7985 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.11 mg/mL (2.11 mM); 澄清溶液

    此方案可获得 ≥ 1.11 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 11.1 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 98.17%

COA (297 KB) HNMR (174 KB) RP-HPLC (299 KB)

产品使用指南 (1538 KB)
参考文献

Physalin F 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8992 mL 9.4961 mL 18.9923 mL 47.4807 mL
5 mM 0.3798 mL 1.8992 mL 3.7985 mL 9.4961 mL
10 mM 0.1899 mL 0.9496 mL 1.8992 mL 4.7481 mL
15 mM 0.1266 mL 0.6331 mL 1.2662 mL 3.1654 mL
20 mM 0.0950 mL 0.4748 mL 0.9496 mL 2.3740 mL
25 mM 0.0760 mL 0.3798 mL 0.7597 mL 1.8992 mL
30 mM 0.0633 mL 0.3165 mL 0.6331 mL 1.5827 mL
40 mM 0.0475 mL 0.2374 mL 0.4748 mL 1.1870 mL
50 mM 0.0380 mL 0.1899 mL 0.3798 mL 0.9496 mL
60 mM 0.0317 mL 0.1583 mL 0.3165 mL 0.7913 mL
80 mM 0.0237 mL 0.1187 mL 0.2374 mL 0.5935 mL
100 mM 0.0190 mL 0.0950 mL 0.1899 mL 0.4748 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Physalin F57423-71-9酸浆苦味素FApoptosisinflammatoryimmunomodulatoryPBMCproliferationcytokineHTLV-1infectionInhibitorinhibitorinhibit

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