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石蜡切片技术

石蜡切片技术是将组织固定、脱水、浸渍石蜡并切割成薄片的方法,用于病理学和组织学研究。首先,组织经过脱水和浸渍,然后嵌入石蜡中,形成坚固的组织块。接着,使用微tome将石蜡包埋组织切成薄片,以便在显微镜下观察。这种技术可以保持组织结构,使其适合组织学切片和免疫组织化学实验,为疾病诊断和生物医学研究提供了可靠的组织学信息。


Paraffin sectioning technique is a method of fixing, dehydrating, paraffin-impregnating and cutting tissues into thin sections for pathological and histological research. First, the tissue is dehydrated and macerated, then embedded in paraffin to form a solid tissue block. Next, use a microtome to cut the paraffin-embedded tissue into thin sections for observation under a microscope. This technology can preserve the tissue structure, making it suitable for histological sectioning and immunohistochemistry experiments, providing reliable histological information for disease diagnosis and biomedical research.

相关实验方案

  • 将包裹在石蜡中的组织通过切片机切成薄片,再进行染色和封片,最后制成可在显微镜下观察的样本。