2. 重组蛋白
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  4. Calnexin 蛋白, Human (HEK293, His)

Calnexin 蛋白, Human (HEK293, His)

目录号: HY-P7711
COA 产品使用指南

Calnexin 蛋白, Human (HEK293, His)是一种由 HEK293 细胞表达的，N 末端有 His 标签的重组人 Calnexin。Calnexin 是一种 90 kDa 的 I 型内质网 (ER) 膜蛋白。

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规格 价格 是否有货 数量
10 μg ￥1150 In-stock
50 μg ￥3450 询价
100 μg   询价  

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描述

Calnexin Protein, Human (HEK293, His), a recombinant human Calnexin produced in HEK293 cells, has a His tag at the N-terminus. Calnexin is a 90 kDa type I endoplasmic reticulum (ER) membrane protein[1].
研究背景

钙连接蛋白和钙网蛋白是构成 ER 伴侣系统的相关蛋白质，可确保新合成的糖蛋白的正确折叠和质量控制[1]。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

P27824 (H21-P481)
基因 ID

821  [NCBI]
蛋白结构
N-term
Calnexin (H21-P481)
Accession # P27824
6*His
C-term
中文名
重组人钙联蛋白, His (HEK293 表达)
同用名
rHuCalnexin, His; Calnexin; IP90; Major Histocompatibility Complex Class I Antigen-Binding Protein p88; CANX
氨基酸序列

HDGHDDDVIDIEDDLDDVIEEVEDSKPDTTAPPSSPKVTYKAPVPTGEVYFADSFDRGTLSGWILSKAKKDDTDDEIAKYDGKWEVEEMKESKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTPELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGIYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSVVNSGNLLNDMTPPVNPSREIEDPEDRKPEDWDERPKIPDPEAVKPDDWDEDAPAKIPDEEATKPEGWLDDEPEYVPDPDAEKPEDWDEDMDGEWEAPQIANPRCESAPGCGVWQRPVIDNPNYKGKWKPPMIDNPSYQGIWKPRKIPNPDFFEDLEPFRMTPFSAIGLELWSMTSDIFFDNFIICADRRIVDDWANDGWGLKKAADGAAEPGVVGQMIEAAEERPHHHHHH

分子量

Approximately 70.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 150 mM NaCl, 2 mM CaCl2, 10% Glycerol, pH 7.5.
内毒素含量

<1 EU/μg, determined by LAL method.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)
参考文献

Calnexin Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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