Calnexin 蛋白, Mouse (HEK293, His)

Calnexin interacts with monoglucosylated glycoproteins, helping their assembly and retention in the endoplasmic reticulum (ER). Calnexin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Calnexin protein, expressed by HEK293 , with C-His labeled tag.

钙连接蛋白是一种位于内质网 (ER) 中的钙结合伴侣，在与新合成的单葡萄糖基化糖蛋白相互作用中发挥着关键作用，有助于蛋白质组装和未组装蛋白质亚基在 ER 内的保留。钙连接蛋白在 ER 质量控制装置中发挥着关键作用，参与保留错误折叠的蛋白质。值得注意的是，它与未成熟胸腺细胞中的部分 T 细胞抗原受体复合物相关，表明在胸腺细胞成熟的信号传导和调节中发挥作用。此外，钙连接蛋白可能参与突触受体介导的内吞作用。其相互作用扩展到多种蛋白质，包括 MAPK3/ERK1、KCNH2、SERPINA2P/SERPINA2、SGIP1、PPIB、SMIM22、TMX2、TMEM35A/NACHO、CHRNA7、RETREG2、RETREG3、DNM1L 和 ADAM7，突出了其在形成复合物和参与不同的细胞过程。棕榈酰化形式与核糖体易位子复合物成分 SSR1 特异性相互作用，促进糖蛋白的有效折叠并强调其在内质网内蛋白质成熟中的作用。

Measured by its ability to enhance the proliferation of SH-SY5Y cells. The ED₅₀ for this effect is 10.92 ng/mL in the presence of 0.5 μg/mL PRNP, corresponding to a specific activity is 9.158×10⁴ units/mg.

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  Measured by its ability to enhance the proliferation of SH-SY5Y cells. The ED₅₀ for this effect is 10.92 ng/mL in the presence of 0.5 μg/mL PRNP, corresponding to a specific activity is 9.158×10⁴ units/mg.

Mouse

HEK293

C-10*His

P35564 (H21-P482)

12330  [NCBI]

HDGHDDDAIDIEDDLDDVIEEVEDSKSKSDASTPPSPKVTYKAPVPTGEVYFADSFDRGSLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKETKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTAELSLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGVYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSVVNSGNLLNDMTPPVNPSREIEDPEDRKPEDWDERPKIADPDAVKPDDWDEDAPSKIPDEEATKPEGWLDDEPEYIPDPDAEKPEDWDEDMDGEWEAPQIANPKCESAPGCGVWQRPMIDNPNYKGKWKPPMIDNPNYQGIWKPRKIPNPDFFEDLEPFKMTPFSAIGLELWSMTSDIFFDNFIISGDRRVVDDWANDGWGLKKAADGAAEPGVVLQMLEAAEERP

Approximately 58-70 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.