CD158d/KIR2DL4 蛋白, Human (HEK293, His)

CD158d/KIR2DL4 Protein, Human (HEK293, His) is a recombinant human CD158d expressed in HEK 293 cells with a His tag at the N-terminus. CD158d/KIR2DL4 Protein is an NK cell-activating receptor with inhibitory potential^[1][2].

KIR2DL4 是 KIR 家族中最独特的基因。KIR2DL4 由 D0 和 D2 Ig 样结构域组成（D0 是 KIR3D 亚家族的第一个 Ig 样结构域）。KIR2DL4 启动子与所有其他 KIR 基因有很大不同，并且两个等位基因基本上在所有活化的 NK 细胞中表达。KIR2DL4 仅在外周血 NK 细胞的 CD56bright 子集的表面上组成型表达。KIR2DL4 与 FcεRIγ 接头蛋白结合，但不与 DAP12 结合，并且在其胞质结构域中具有功能性 ITIM。尽管存在 ITIM，KIR2DL4 与 mAb 交联仍会诱导静息 NK 细胞产生 IFN-γ，并触发 IL-2 激活的 NK 细胞产生细胞毒性和 IFN-γ^{[1] [2]}。

Human

HEK293

C-6*His

ADY38409.1 (W22-H242)

3805  [NCBI]

WAHVGGQDKPFCSAWPSAVVPQGGHVTLRCHYRRGFNIFTLYKKDGVPVPELYNRIFWNSFLISPVTPAHAGTYRCRGFHPHSPTEWSAPSNPLVIMVTGLYEKPSLTARPGPTVRTGENVTLSCSSQSSFDIYHLSREGEAHELRLPAVPSINGTFQADFPLGPATHGETYRCFGSFHGSPYEWSDASDPLPVSVTGNPSSSWPSPTEPSFKTGIARHLH

Approximately 30-40 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Faure M, et, al. KIR2DL4 (CD158d), an NK cell-activating receptor with inhibitory potential. J Immunol. 2002 Jun 15;168(12):6208-14.  [Content Brief]

  [2]. Lanier LL. NK cell recognition. Annu Rev Immunol. 2005;23:225-74.  [Content Brief]