CD36 蛋白, Rat (HEK293, His)

CD36 is a multifunctional glycoprotein that serves as a receptor for a variety of ligands, including thrombospondin, collagen, and oxidized low-density lipoprotein. Its multivalent ligand induces CD36 clusters, initiating signal transduction. CD36 Protein, Rat (HEK293, His) is the recombinant rat-derived CD36 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD36 Protein, Rat (HEK293, His) is 410 a.a., with molecular weight of 70-80 kDa.

CD36 是一种多功能糖蛋白，作为多种配体的受体，包括血小板反应蛋白、纤连蛋白、胶原蛋白或淀粉样蛋白等蛋白质实体，以及氧化低密度脂蛋白 (oxLDL)、阴离子磷脂、长链磷脂等脂质成分。链脂肪酸和细菌二酰化脂肽。由于其多价性质，这些配体可以同时与多个受体结合，促进 CD36 簇的形成，从而启动信号转导和受体-配体复合物的内化。随后的细胞反应在血管生成、炎症反应、脂肪酸代谢以及肠道中味觉和膳食脂肪的处理中发挥着关键作用。CD36 参与结合长链脂肪酸，促进其转运到细胞中，有助于肌肉脂质利用、脂肪能量储存和肠道脂肪吸收。从机制上讲，脂肪酸结合激活下游激酶 LYN，导致棕榈酰转移酶 ZDHHC5 磷酸化和失活，随后导致 CD36 去棕榈酰化和小凹胞吞作用。在小肠中，CD36 可能通过激活 MAPK1/3 (ERK1/2) 信号通路参与膳食脂肪酸和胆固醇的近端吸收。此外，CD36 对于口腔脂肪感知和偏好至关重要，它存在于味觉受体细胞中，介导细胞内钙水平增加并激活味觉神经元。在下丘脑腹内侧区，CD36 在感知长链脂肪酸以及调节能量和葡萄糖稳态方面发挥着关键作用。CD36 作为血小板反应蛋白的受体和 TLR4:TLR6 异二聚体的辅助受体，分别促进单核细胞/巨噬细胞的抗血管生成作用和炎症。配体结合后，CD36 与 TLR4:TLR6 异二聚体相互作用，导致内化并通过 NF-κ-B 依赖性途径引发炎症反应。此外，CD36 作为微生物二酰化脂肽的选择性和非冗余传感器，引发 NF-κ-B 依赖性细胞因子的产生，并随后通过 TLR2:TLR6 异二聚体信号传导靶向脂筏依赖性途径中的高尔基体。

Immobilized Rat CD36, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC₅₀ of 7.9 ng/mL determined by ELISA.

Rat

HEK293

C-His

Q07969 (G30-K439)

29184  [NCBI]

GDLLIEKTIKREVVLEEGTIAFKNWVKTGTTVYRQFWIFDVQNPEEVAKNSSKIKVKQRGPYTYRVRYLAKENITQDPKDSTVSFVQPNGAIFEPSLSVGTENDNFTVLNLAVAAAPHIYTNSFVQGVLNSLIKKSKSSMFQTRSLKELLWGYKDPFLSLVPYPISTTVGVFYPYNNTVDGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESYCDMINGTDAASFPPFVEKSQTLRFFSSDICRSIYAVFESEVNLKGIPVYRFVLPANAFASPLQNPDNHCFCTEKVISNNCTSYGVLDIGKCKEGKPVYISLPHFLHASPDVSEPIEGLNPNEDEHRTYLDVEPITGFTLQFAKRLQVNILVKPARKIEALKNLKRPYIVPILWLNETGTIGDEKAEMFRNQVTGKIK

70-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.