CHGA 蛋白, Human (P.pastoris, His)

CHGA protein robustly inhibits glucose-induced insulin release from the pancreas, functioning as a non-competitive nicotinic cholinergic antagonist. It inhibits catecholamine release, displays antibacterial activity, induces mast cell responses, and scavenges free radicals. CHGA may also regulate cardiac function and blood pressure. CHGA Protein, Human (P.pastoris, His) is the recombinant human-derived CHGA protein, expressed by P. pastoris , with N-His labeled tag. The total length of CHGA Protein, Human (P.pastoris, His) is 439 a.a., with molecular weight of ~50.9 kDa.

CHGA 蛋白对葡萄糖诱导的胰腺胰岛素释放具有强烈的抑制作用。它作为非竞争性烟碱胆碱能拮抗剂，抑制嗜铬细胞和去甲肾上腺素能神经元释放儿茶酚胺。此外，CHGA 对革兰氏阳性菌（金黄色葡萄球菌和藤黄杆菌）和革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）均具有抗菌活性。此外，它具有诱导肥大细胞迁移、脱颗粒以及细胞因子和趋化因子产生的能力。此外，CHGA 在体外可作为自由基的有效清除剂，并可能在调节心脏功能和血压中发挥作用。

Human

P. pastoris

N-His

P10645 (L19-G457)

1113  [NCBI]

LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG

Approximately 50.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.