CHST5 蛋白, Mouse (HEK293, Fc)

CHST5 protein, utilizing PAPS, catalyzes sulfate transfer to position 6 of non-reducing GlcNAc residues in keratan, particularly in the cornea. This sulfonation is crucial for mediating keratan sulfate sulfation, a key process in maintaining corneal transparency. CHST5 acts on short and long carbohydrate substrates, emphasizing its role in corneal function and contribution to maintaining corneal transparency through keratan sulfate sulfation. CHST5 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CHST5 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CHST5 Protein, Mouse (HEK293, Fc) is 369 a.a., with molecular weight of 75-80 KDa.

CHST5 蛋白是一种利用 3'-磷酸-5'-腺苷基硫酸盐 (PAPS) 作为磺酸盐供体的磺基转移酶，在催化硫酸盐转移到角质素非还原性 N-乙酰葡糖胺 (GlcNAc) 残基的 6 位中发挥着关键作用，特别是在角膜中。这种磺化过程对于介导角质素的硫酸化至关重要，角质素是维持角膜透明度的关键成分。CHST5 作用于具有聚 N-乙酰基乳糖胺结构的短碳水化合物和长碳水化合物底物，靶向非还原末端 GlcNAc。此外，这种磺基转移酶可能对粘蛋白型受体的 O-连接糖表现出活性。CHST5 的特定酶作用强调了其在涉及角膜功能的复杂过程中的重要性，强调了其在硫酸角质素硫酸化中的作用及其对维持角膜透明度的贡献。

Measured by its ability to transfer sulfate from PAPS to N-acetyl-D-glucosamine. The specific activity is 2765.67 pmol/min/μg that incubate at 37 ºC for 20 minutes.

Mouse

HEK293

N-hFc

Q9QUP4 (S27-S395)

56773  [NCBI]

SRQVPSSPAGLGERVHVLVLSSWRSGSSFVGQLFSQHPDVFYLMEPAWHVWDTLSQGSAPALHMAVRDLIRSVFLCDMDVFDAYLPWRRNISDLFQWAVSRALCSPPVCEAFARGNISSEEVCKPLCATRPFGLAQEACSSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAVLRSREQTAKALARDNGIVLGTNGTWVEADPRLRVVNEVCRSHVRIAEAALHKPPPFLQDRYRLVRYEDLARDPLTVIRELYAFTGLGLTPQLQTWIHNITHGSGPGARREAFKTTSRDALSVSQAWRHTLPFAKIRRVQELCGGALQLLGYRSVHSELEQRDLSLDLLLPRGMDSFKWASSTEKQPES

Approximately 75-80 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.