1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Complement factor I 蛋白, Mouse (HEK293, His)

Complement factor I 蛋白, Mouse (HEK293, His)

目录号: HY-P703770
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Complement factor I 蛋白, Mouse (HEK293, His) 是重组小鼠来源的 Complement factor I 蛋白,由 HEK293 表达,带有 C-His 标签。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
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100 μg   询价  

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描述

Complement factor I Protein, Mouse (HEK293, His) is the recombinant mouse-derived Complement factor I protein, expressed by HEK293, with C-His tag.

研究背景

Complement factor I 是一种 trypsin-like serine protease,在调控免疫反应中发挥关键作用,通过控制所有补体途径(complement pathways)来抑制过度激活。它通过切割 C3b 和 C4b 的 α 链(分别切割 3 个和 2 个肽键)使其失活,从而阻断补体级联反应。该过程需要辅助因子参与:液相中的 factor H 和 C4BP,以及细胞膜表面的 membrane cofactor protein/CD46 和 CR1。在健康细胞上,这些辅助因子的存在允许 CFI 降解沉积的 C3b,避免异常补体激活;而在凋亡细胞或微生物中,辅助因子的缺失会导致 C3b 介导的补体激活及后续调理作用(opsonization)。by similar: 功能描述基于对补体调控机制的共性归纳。

种属

Mouse

表达系统

HEK293

标签

C-8*His

蛋白编号

Q61129 (R19-V603)

蛋白长度

Full Length of Mature Protein

中文名
补体因子 I 蛋白
同用名
Complement factor I; C3B/C4B inactivator; Cfi
预测分子量
66.4 kDa
分子量

Approximately 80-100 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

糖基化修饰
Yes
纯度

Greater than 90% as determined by reducing SDS-PAGE.;> 90% as determined by HPLC

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Complement factor I Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Complement factor I Protein, Mouse (HEK293, His)
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