Copine-1/CPNE1 蛋白, Human (His)

Copine-1/CPNE1 protein is a calcium-dependent phospholipid-binding protein that participates in the TNF-α receptor signaling pathway in a calcium-dependent manner. CPNE1 induces AKT-dependent neurite outgrowth and is also involved in TNF-α-induced repression of NF-κ-B transcription. Copine-1/CPNE1 Protein, Human (His) is the recombinant human-derived Copine-1/CPNE1 protein, expressed by E. coli , with N-6*His, C-6*His labeled tag. The total length of Copine-1/CPNE1 Protein, Human (His) is 537 a.a., with molecular weight of 58-66 kDa.

Copine-1，也称为 CPNE1，是一种钙依赖性磷脂结合蛋白，积极参与钙介导的细胞内过程。Copine-1 参与 TNF-α 受体信号传导通路，表现出钙依赖性磷脂结合特性。此外，它通过 AKT 依赖性信号级联以不依赖钙的方式作用，诱导神经突生长，从而在神经祖细胞分化中发挥关键作用。Copine-1 还可能以钙依赖性方式促进靶蛋白向细胞膜的募集，并参与膜运输。此外，它通过诱导各种 NF-kappa-B 亚基（包括 p65/RELA、p50/NFKB1、p52/NFKB2、RELB 和 REL）的内切蛋白酶加工，参与 TNF-α 诱导的 NF-kappa-B 转录抑制。Copine-1 的多方面功能凸显了其在细胞过程中的多功能作用，从神经元发育到膜动力学和信号转导途径。

Human

E. coli

N-6*His;C-6*His

Q99829 (M1-A537)

8904  [NCBI]

MAHCVTLVQLSISCDHLIDKDIGSKSDPLCVLLQDVGGGSWAELGRTERVRNCSSPEFSKTLQLEYRFETVQKLRFGIYDIDNKTPELRDDDFLGGAECSLGQIVSSQVLTLPLMLKPGKPAGRGTITVSAQELKDNRVVTMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPSTPIQVQCSDYDSDGSHDLIGTFHTSLAQLQAVPAEFECIHPEKQQKKKSYKNSGTIRVKICRVETEYSFLDYVMGGCQINFTVGVDFTGSNGDPSSPDSLHYLSPTGVNEYLMALWSVGSVVQDYDSDKLFPAFGFGAQVPPDWQVSHEFALNFNPSNPYCAGIQGIVDAYRQALPQVRLYGPTNFAPIINHVARFAAQAAHQGTASQYFMLLLLTDGAVTDVEATREAVVRASNLPMSVIIVGVGGADFEAMEQLDADGGPLHTRSGQAAARDIVQFVPYRRFQNAPREALAQTVLAEVPTQLVSYFRAQGWAPLKPLPPSAKDPAQAPQA

58-66 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB，150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.