CSF1R 蛋白, Mouse (HEK293, Fc)

The CSF1R protein is a tyrosine protein kinase receptor for CSF1 and IL34 and plays a critical regulatory role in hematopoietic cells, especially mononuclear phagocytes. Its effects span innate immunity, inflammation, osteoclast function, skeletal development, and fertility. CSF1R Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CSF1R protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CSF1R Protein, Mouse (HEK293, Fc) is 492 a.a., with molecular weight of ~81.9 kDa.

CSF1R 蛋白是一种酪氨酸蛋白激酶，作为 CSF1 和 IL34 的细胞表面受体，对造血前体细胞（尤其是巨噬细胞和单核细胞等单核吞噬细胞）的生存、增殖和分化发挥关键控制作用。它在先天免疫和炎症过程中的作用通过其响应 IL34 和 CSF1 促进促炎趋化因子的释放而得到强调。此外，CSF1R在破骨细胞增殖和分化、骨吸收的调节中发挥着关键作用，是正常骨骼和牙齿发育所不可缺少的。它对于男性和女性的正常生育能力以及怀孕期间乳腺中乳管和腺泡结构的发育至关重要。值得注意的是，CSF1R 影响肌动蛋白细胞骨架的重组，调节膜皱褶的形成、细胞粘附、细胞迁移，并促进癌细胞侵袭。配体结合后，CSF1R 激活多种信号通路，包括 ERK1/2、JNK、PI3K/AKT、MAP 激酶（MAPK1/ERK2 和/或 MAPK3/ERK1）和 SRC 家族激酶（SRC、FYN、YES1）。其下游效应涉及多种靶蛋白的磷酸化，例如 PIK3R1、PLCG2、GRB2、SLA2 和 CBL。此外，CSF1R 介导 STAT 家族成员（STAT3、STAT5A 和/或 STAT5B）的激活，并促进 SHC1 和 INPP5D/SHIP-1 的酪氨酸磷酸化。受体信号传导受到蛋白磷酸酶（包括 INPP5D/SHIP-1）以及激活受体的快速内化的严格调节。在中枢神经系统中，CSF1R 可能有助于小胶质细胞巨噬细胞的发育。

Measured by its ability to inhibit the mouse CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is typically 0.1-0.7 μg/mL in the presence of 15 ng/mL mouse M-CSF.

Mouse

HEK293

C-hFc

P09581 (A20-S511)

12978  [NCBI]

MELGPPLVLLLATVWHGQGAPVIEPSGPELVVEPGETVTLRCVSNGSVEWDGPISPYWTLDPESPGSTLTTRNATFKNTGTYRCTELEDPMAGSTTIHLYVKDPAHSWNLLAQEVTVVEGQEAVLPCLITDPALKDSVSLMREGGRQVLRKTVYFFSPWRGFIIRKAKVLDSNTYVCKTMVNGRESTSTGIWLKVNRVHPEPPQIKLEPSKLVRIRGEAAQIVCSATNAEVGFNVILKRGDTKLEIPLNSDFQDNYYKKVRALSLNAVDFQDAGIYSCVASNDVGTRTATMNFQVVESAYLNLTSEQSLLQEVSVGDSLILTVHADAYPSIQHYNWTYLGPFFEDQRKLEFITQRAIYRYTFKLFLNRVKASEAGQYFLMAQNKAGWNNLTFELTLRYPPEVSVTWMPVNGSDVLFCDVSGYPQPSVTWMECRGHTDRCDEAQALQVWNDTHPEVLSQKPFDKVIIQSQLPIGTLKHNMTYFCKTHNSVGNSSQYFRAVSLGQSKQLPDES

Approximately 81.9 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.