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  4. CSNK1A1 蛋白, Human (sf9, His, Strep)

CSNK1A1 蛋白, Human (sf9, His, Strep)

目录号: HY-P702668
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酪蛋白激酶 I α 是酪蛋白激酶的一员,表现出对酸性蛋白(例如酪蛋白)的磷酸化的偏好。它磷酸化许多底物,包括参与 Wnt 信号传导的“Ser-45”位点的 CTNNB1。CSNK1A1 蛋白, Human (sf9, His, Strep) 是重组的 CSNK1A1 蛋白,由 Sf9 insect cells 表达,带有 Strep, His 标签。CSNK1A1 蛋白, Human (sf9, His, Strep) 全长 336 个氨基酸

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描述

Casein kinase I α is a member of casein kinases that exhibits a preference for phosphorylation of acidic proteins such as casein. It phosphorylates many substrates, including CTNNB1 at "Ser-45" involved in Wnt signaling. CSNK1A1 Protein, Human (sf9, His, Strep) is the recombinant human-derived CSNK1A1, expressed by Sf9 insect cells , with Strep, His labeled tag. The total length of CSNK1A1 Protein, Human (sf9, His, Strep) is 336 a.a.,

研究背景

酪蛋白激酶 I α 是酪蛋白激酶家族的成员,其特点是偏爱酸性底物,例如酪蛋白。它具有多功能性,可磷酸化多种蛋白质,并通过磷酸化 CTNNB1 的“Ser-45”位点积极参与 Wnt 信号传导。此外,酪蛋白激酶 I α 可能有助于 PER1 和 PER2 的磷酸化,表明其在昼夜节律调节中具有潜在作用。除了参与细胞信号传导途径之外,该激酶可能在有丝分裂过程中的染色体分离中发挥至关重要的作用,并通过影响角蛋白细胞骨架的分解来调节上皮细胞迁移。此外,酪蛋白激酶 I α 作为 mTORC1mTORC2 信号的正调节因子响应营养刺激,介导 DEPTOR 抑制剂的磷酸化。在另一种情况下,它通过 NLRP3 的磷酸化充当 NLRP3 炎性体组装的抑制剂,突出了其在细胞过程中的多种作用。

种属

Human

表达系统

Sf9 insect cells

标签

Strep;His

蛋白编号

P48729-1 (A2-F337)

基因 ID

1452

同用名
CSNK1A1; casein kinase 1, alpha 1; casein kinase I isoform alpha; CK1; CK1a; CK1alpha; CKIa; CKIalpha; clock regulator kinase; CKI-alpha; down-regulated in lung cancer; HLCDGP1; PRO2975
纯度

Greater than 80 % as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CSNK1A1 Protein, Human (sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CSNK1A1 Protein, Human (sf9, His, Strep)
目录号:
HY-P702668
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