1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. DNASE1L3 蛋白, Human (His)

DNASE1L3 蛋白, Human (His)

目录号: HY-P703079
产品使用指南

DNASE1L3 通过蛋白水解切割单链和双链 DNA,并生成带有 3'-OH 末端的片段。它可以切割染色质、核小体和脂质体包被的 DNA。DNASE1L3 蛋白, Human (His) 是重组的 DNASE1L3 蛋白,由 E. coli 表达,带有 C-10*His 标签。

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描述

DNASE1L3 proteolytically cleaves single- and double-stranded DNA and generates fragments with 3'-OH termini. It can cleave chromatin, nucleosomes, and liposome-coated DNA. DNASE1L3 Protein, Human (His) is the recombinant human-derived DNASE1L3 protein, expressed by E. coli , with C-10*His labeled tag.

研究背景

DNASE1L3 蛋白具有 DNA 水解活性,能够熟练地切割单链和双链 DNA,从而产生带有 3'-OH 末端的 DNA 片段。该蛋白质可以将染色质切割成核小体单位,并对核小体和脂质体包被的 DNA 表现出活性。它在细胞凋亡和坏死过程中的核小体间 DNA 断裂中发挥着至关重要的作用,有助于肌源性和神经元分化等过程,以及 BCR 介导的自身反应性 B 细胞的克隆缺失。DNASE1L3 对凋亡细胞来源的膜包被微粒中的染色质具有活性,从而抑制抗 DNA 自身免疫。此外,DNASE1L3 与 DNASE1 合作,对于降解中性粒细胞胞外陷阱 (NET) 至关重要,NET 主要由中性粒细胞在炎症过程中释放的 DNA 纤维组成。DNASE1 和 DNASE1L3 对血管内 NET 的降解对于防止血栓形成至关重要,血栓可能会阻塞血管并导致炎症后的器官损伤。

种属

Human

表达系统

E. coli

标签

C-10*His

蛋白编号

Q13609 (M21-S305)

基因 ID

1776

蛋白结构
N-term
DNASE1L3 (M21-S305)
Accession # Q13609
10*His
C-term
同用名
Deoxyribonuclease gamma; Deoxyribonuclease I like 3; Deoxyribonuclease I like III; Deoxyribonuclease I-like 3; DHP 2; DHP2; DNAS1L3; DNase gamma; DNase I homolog protein 2; DNase I homolog protein DHP2; DNase I like 3; DNase I-like 3; DNASE1L3; DNSL3_HUMAN; Liver and spleen DNase; LS DNase; LS-DNase; LSD; SLEB 16; SLEB16
氨基酸序列

MRICSFNVRSFGESKQEDKNAMDVIVKVIKRCDIILVMEIKDSNNRICPILMEKLNRNSRRGITYNYVISSRLGRNTYKEQYAFLYKEKLVSVKRSYHYHDYQDGDADVFSREPFVVWFQSPHTAVKDFVIIPLHTTPETSVKEIDELVEVYTDVKHRWKAENFIFMGDFNAGCSYVPKKAWKNIRLRTDPRFVWLIGDQEDTTVKKSTNCAYDRIVLRGQEIVSSVVPKSNSVFDFQKAYKLTEEEALDVSDHFPVEFKLQSSRAFTNSKKSVTLRKKTKSKRS

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

DNASE1L3 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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