2. 重组蛋白
  3. Receptor Proteins
  4. G-Protein-Coupled Receptors (GPCRs)
  5. Endothelin Receptor
  6. Endothelin Receptor Type A (EDNRA)
  7. EDNRA Protein-VLP, Human (HEK293)

EDNRA Protein-VLP, Human (HEK293)

目录号: HY-P704570
产品使用指南 技术支持

EDNRA 蛋白是内皮素 1 的受体，通过与 G 蛋白结合激活磷脂酰肌醇-钙第二信使系统。其结合亲和力遵循以下顺序：ET1 > ET2 >> ET3。此外，EDNRA 与 HDAC7 和 KAT5 相互作用。EDNRA Protein-VLP, Human (HEK293) 是重组的 EDNRA 蛋白，由 HEK293 表达，不带标签。VLPs在HEK293细胞培养中形成，将膜蛋白集中在细胞表面，产生适合免疫和抗体筛选的高浓度蛋白。VLPs免疫原性强，需优化免疫策略，包括抗原剂量、接种方案和佐剂选择。本产品不推荐应用于受配体互作实验和SPR/BLI分析, 因为VLP包膜上还有宿主其他无关膜蛋白, 受配体互作会有很强的背景干扰。SPR/BLI分析则对芯片和实验方案有较高要求，成功率较低。如果需要VLP对照，推荐HY-P705433。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
20 μg ￥7350 1 - 2 周
100 μg ￥14700 1 - 2 周
> 100 μg   询价  

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描述

EDNRA Protein, a receptor for endothelin-1, activates a phosphatidylinositol-calcium second messenger system through association with G proteins. Its binding affinities follow the order: ET1 > ET2 >> ET3. Additionally, EDNRA interacts with HDAC7 and KAT5. EDNRA Protein-VLP, Human (HEK293) is the recombinant human-derived EDNRA protein, expressed by HEK293, with tag free.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.
研究背景

EDNRA 是细胞信号传导的关键参与者，作为内皮素-1 的受体发挥作用，通过 G 蛋白执行其作用并启动磷脂酰肌醇-钙第二信使系统。该受体表现出不同的结合亲和力，其中 ET1 具有最高的亲和力，其次是 ET2 和 ET3。除了在内皮素信号转导中的典型功能外，EDNRA 还参与分子相互作用，与 HDAC7 和 KAT5 形成复合物，可能有助于复杂的细胞调节网络。内皮素-1 信号传导和蛋白质相互作用的融合凸显了 EDNRA 在协调不同细胞过程中的多方面作用。
种属

Human
表达系统

HEK293
标签

Tag Free
蛋白编号

P25101 (D21-D427)
基因 ID

1909
蛋白结构
N-term
EDNRA (D21-D427)
Accession # P25101
C-term
同用名
Ednra; ET A; ET-A; ETA; ETA R; ETA-R; ETAR; ETRA; G protein coupled receptor; hET AR; hET-AR; MFDA
性状

溶液.
组分

Supplied as 0.2 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料
产品使用指南 (1213 KB)

EDNRA Protein-VLP, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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