EGF 蛋白, Human

EGF Protein is a potent epidermal growth factor that belongs to EGF-family of proteins. EGF Protein stimulates cell growth and differentiation by binding to epidermal growth factor receptor (EGFR) found in many human tissues, including platelets, submandibular gland (submaxillary gland) and parotid gland. EGF Protein has effects such as wound healing, anti-intestinal atrophy, anti-cancer, reducing ureteral obstruction and assisting in nerve tissue regeneration, mainly used in wound healing applications. EGF Protein (Human) is a recombinant protein with tag free that consists of 53 amino acids with three intramolecular disulfide bonds and is produced in E. coli^{[1],[2],[3],[4],[5],[6],[7]}.

重组人表皮生长因子的化学成分与天然材料相同，具有完整的生物活性，可用于伤口愈合应用[1]。重组人表皮生长因子 (rhEGF) 刺激成纤维细胞 BALB/c3T3 细胞系的增殖。从水凝胶中释放的重组人表皮生长因子可保持其生物活性，诱导 EGF 受体表达，引起细胞核抗原增殖，并在促进糖尿病伤口愈合方面显示出治疗潜力^[1,2]。
EGF 蛋白 (Human) 通过与细胞表面的表皮生长因子受体 (EGFR) 高亲和力结合，促进细胞增殖，分化和存活^[1]。
唾液 EGF 蛋白 (Human) 的生物效应包括促进口腔和胃食管溃疡愈合，抑制胃酸分泌，刺激 DNA 合成，以及保护粘膜免受胃酸，胆汁酸，胃蛋白酶和胰蛋白酶等腔内有害因素，以及物理，化学和细菌的损害^[3]。
EGF 蛋白 (Human) 在骨髓间充质干细胞 (MSCs) 分化过程中起矿化剂作用。EGF 蛋白 (Human) 能够增加钙沉积的形成以及 ALP和 OCN 基因的表达，有望用于促进牙周病和口腔种植骨再生的有效辅助剂的研究^[4,5]。

EGF 蛋白 (Human) (10 ng/mL, 7 天) 使牙髓干细胞 (DPSCs) 呈多边形状，外周核呈球形，胞质含量低，对 DPSCs 中 CD146 和 CD10 的表达水平有较强的抑制作用^[4]。
EGF 蛋白 (Human) (10 ng/mL, 21 天) 明显提高了钙沉积的数量，大小和通过 Alizarin red S (ARS) (HY-120601) (1686.31 μg/mL) 评估的矿化水平^[4]。
EGF 蛋白 (Human) 显著增强人间充质干细胞 (hMSC) 中酪氨酸磷酸化，碱性磷酸酶活性和矿化结节形成^[4]。
EGF 蛋白 (Human) (10 ng/μL，21 天) 促进了 Sox10 介导的神经胶质细胞命运转变为寡突胶质细胞前体 (iOPCs)，这些前体能够进一步分化为髓鞘化的寡突胶质细胞 (OLs)^[7]。

EGF 蛋白 (Human) (10 μg/g, 乳膏, 单次剂量, 持续 7 天）在全层皮肤创口的大鼠中可以减少炎症浸润，并促使伤口再上皮化^[1]。
EGF 蛋白 (Human) (50 μg/g，乳膏，每 24 小时，连用 7 天) 可显著刺激有背部厚创伤猪的再上皮细胞形成，最好与蛋白酶抑制剂联合使用^[1]。
EGF 蛋白 (Human) (30 μg/kg, 皮下注射, 每天注射, 持续 3 周) 可以减轻戈廷根迷你猪中由硬化疗法 (食道的外科扩张) 导致的食道损伤^[1]。

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED₅₀ for this effect is <300 pg/mL.

• 
  Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.The ED₅₀ for this effect is 53.08 pg/mL, corresponding to a specific activity is 1.88×10⁷ units/mg.

Human

E. coli

Tag Free

P01133-1 (N971-R1023)

1950  [NCBI]

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

Approximately 7-10 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 10 mM Phosphate buffer, pH 7.0, 200 mM NaCl or 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM Tris, 200 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 200 mM NaCl, pH 8.0 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wong WR, et al. Applications, and efficient large-scale production, of recombinant human epidermal growth factor. Biotechnol Genet Eng Rev. 2001;18:51-71.  [Content Brief]

  [2]. Lao G, et al. Controlled release of epidermal growth factor from hydrogels accelerates wound healing in diabetic rats. J Am Podiatr Med Assoc. 2012 Mar-Apr;102(2):89-98.  [Content Brief]

  [3]. Venturi S, et al. Iodine in evolution of salivary glands and in oral health. Nutr Health. 2009;20(2):119-34.  [Content Brief]

  [4]. Del Angel-Mosqueda C, et al. Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro. Head Face Med. 2015 Sep 3;11:29.  [Content Brief]

  [5]. Kratchmarova I, et al. Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation. Science. 2005 Jun 3;308(5727):1472-7.  [Content Brief]

  [6]. Li X, et al. Common and rare variants of EGF increase the genetic risk of Alzheimer's disease as revealed by targeted sequencing of growth factors in Han Chinese. Neurobiol Aging. 2023 Mar;123:170-181.  [Content Brief]

  [7]. Liu X, et al. EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes. Mol Med. 2022 May 4;28(1):50.  [Content Brief]