EGF 蛋白, Mouse

EGF Protein, Mouse is a growth factor that stimulates the proliferation of the epidermis and several epithelial tissues.

表皮生长因子 (EGF) 与表皮生长因子受体结合，刺激细胞内信号转导级联，从而激活调节细胞增殖、血管生成、运动和转移的基因^[1]。表皮生长因子 (EGF) 最初是作为 1217 个氨基酸的大前体合成的，经过糖基化，可以被细胞分泌。表皮生长因子 (EGF) mRNA 和蛋白质在许多成人组织中表达，特别是在胃肠道的上皮细胞中。该肽的主要合成部位是颌下腺、小肠中的布伦纳腺和肾脏^[2]。

The ED₅₀ is <0.1 ng/mL as measured by BALB/c 3T3 cells, corresponding to a specific activity of >1.0 × 10⁷ units/mg.

Mouse

E. coli

Tag Free

P01132 (N977-R1029)

13645  [NCBI]

NSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELR

Approximately 8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hynes NE, et al. ERBB receptors and cancer: the complexity of targeted inhibitors. Nat Rev Cancer. 2005 May;5(5):341-54.  [Content Brief]

  [2]. Salomon DS, et al. Epidermal growth factor-related peptides and their receptors in human malignancies. Crit Rev Oncol Hematol. 1995 Jul;19(3):183-232.  [Content Brief]