1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. EIF4A1 蛋白, Human (His, Strep)

EIF4A1 蛋白, Human (His, Strep)

目录号: HY-P701606
产品使用指南

EIF4A1蛋白是一种ATP依赖性RNA解旋酶,是eIF4F复合物的重要亚基,对于帽子识别和促进mRNA与核糖体的结合至关重要。在翻译起始模型中,EIF4A1 有助于解开 mRNA 5'-UTR 中的 RNA 二级结构。EIF4A1 蛋白, Human (His, Strep) 是重组的 EIF4A1 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。EIF4A1 蛋白, Human (His, Strep) 全长 406 个氨基酸。

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描述

The EIF4A1 protein is an ATP-dependent RNA helicase and an important subunit of the eIF4F complex, which is essential for cap recognition and promoting the binding of mRNA to ribosomes. In the translation initiation model, EIF4A1 helps unravel RNA secondary structure in the mRNA 5'-UTR. EIF4A1 Protein, Human (His, Strep) is the recombinant human-derived EIF4A1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of EIF4A1 Protein, Human (His, Strep) is 406 a.a., .

研究背景

EIF4A2 是一种 ATP 依赖性 RNA 解旋酶,作为 eIF4F 复合物的关键亚基,在帽子识别和促进 mRNA 与核糖体结合方面发挥关键作用。在流行的翻译起始模型中,EIF4A2 有助于解开 mRNA 5'-UTR 中存在的 RNA 二级结构。这种解旋活性对于小核糖体亚基的有效结合以及随后扫描起始密码子(翻译起始的基本过程)至关重要。

种属

Human

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

P60842 (M1-I406)

基因 ID

1973

蛋白结构
N-term
Strep-6*His
EIF4A1 (M1-I406)
Accession # P60842
C-term
同用名
EIF4A1; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

EIF4A1 Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EIF4A1 Protein, Human (His, Strep)
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