EIF4EBP1 蛋白, Human (His)

EIF4EBP1 is a translation initiation repressor protein that complexly regulates EIF4E activity. In the hypophosphorylated state, EIF4EBP1 competes with EIF4G1/EIF4G3 to inhibit translation by binding to EIF4E. EIF4EBP1 Protein, Human (His) is the recombinant human-derived EIF4EBP1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of EIF4EBP1 Protein, Human (His) is 118 a.a., with molecular weight of ~17.0 kDa.

EIF4EBP1 是一种翻译起始阻遏蛋白，通过调节 EIF4E 与 eIF4F 复合物的关联来复杂地调节 EIF4E 活性。在低磷酸化状态下，EIF4EBP1 与 EIF4G1/EIF4G3 激烈竞争与 EIF4E 的结合，从而抑制翻译。相反，过度磷酸化形式与 EIF4E 分离，促进 EIF4G1/EIF4G3 和 EIF4E 之间的相互作用，从而启动翻译。EIF4EBP1 作为蛋白质翻译调节中的关键介质，对来自激素、生长因子以及通过 MAP 激酶和 mTORC1 途径起作用的刺激的信号做出反应。胰岛素刺激的 MAP 激酶或 mTORC1 磷酸化事件与 EIF4EBP1 动态之间的相互作用强调了其在微调细胞对不同信号线索的反应中的作用。此外，EIF4EBP1 与 RPTOR 的相互作用，特别是通过 TOS 基序，进一步凸显了它参与 mTORC1 介导的磷酸化过程。

Human

E. coli

N-6*His

Q13541 (M1-I118)

1978  [NCBI]

MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI

Approximately 17.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.