2. 重组蛋白
  3. FASN 蛋白, Human (Sf9, His, Strep, Flag)

FASN 蛋白, Human (Sf9, His, Strep, Flag)

目录号: HY-P703075
产品使用指南

FASN（脂肪酸合成酶）是一种多功能酶，是使用 NADPH 从乙酰辅酶 A 和丙二酰辅酶 A 中从头生物合成长链饱和脂肪酸的关键。这种多功能蛋白含有七种催化活性，并具有酰基载体蛋白 (ACP) 结构域和辅基 4'-磷酸泛硫氨酸的结合位点。FASN 蛋白, Human (Sf9, His, Strep, Flag) 是重组的 FASN 蛋白，由 Sf9 insect cells 表达，带有 N-His, N-Strep, C-Flag 标签。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

FASN (fatty acid synthase) is a multifunctional enzyme that is key to the de novo biosynthesis of long-chain saturated fatty acids from acetyl-CoA and malonyl-CoA using NADPH. This multifunctional protein contains seven catalytic activities and has an acyl carrier protein (ACP) domain with a binding site for the prosthetic group 4'-phosphopantetheine. FASN Protein, Human (Sf9, His, Strep, Flag) is the recombinant human-derived FASN protein, expressed by Sf9 insect cells , with N-His, N-Strep, C-Flag labeled tag.
研究背景

脂肪酸合酶 (FASN) 是一种多功能酶，对于长链饱和脂肪酸的从头生物合成至关重要，在 NADPH 存在下利用乙酰辅酶 A 和丙二酰辅酶 A。这种多功能蛋白质具有七种催化活性和酰基载体蛋白 (ACP) 结构域的辅基 4'-磷酸泛硫氨酸的结合位点。值得注意的是，FASN 在 SARS 冠状病毒-2 (SARS-CoV-2)（导致 COVID-19 的病毒）的复制中发挥着关键作用，因为其酶活性是病毒复制过程所必需的。
种属

Human
表达系统

Sf9 insect cells
标签

N-His;C-Flag;N-Strep
蛋白编号

P49327 (E2-G2511)
基因 ID

2194
蛋白结构
N-term
His-Flag
FASN (Q1109-G1524)
Accession # P49327
Flag
C-term
同用名
FASN; Fatty acid synthase; Type I fatty acid synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; 3-oxoacyl-[acyl-carrier-protein] synthase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; Enoyl-[acyl-carrier-protein] reductase; Acyl-[acyl-carrier-protein] hydrolase
分子量

277.5 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称：
FASN Protein, Human (Sf9, His, Strep, Flag)
目录号：
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