1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. FNTA 蛋白, Human

FNTA 蛋白, Human

目录号: HY-P701885
产品使用指南

FNTA蛋白是法尼基转移酶和香叶基香叶基转移酶复合物中的关键亚基,它可以将法尼基或香叶基香叶基部分转移到蛋白质中具有Cys-脂肪族-脂肪族-X序列半胱氨酸残基的蛋白质上。这种酶活性有助于翻译后蛋白质修饰。FNTA 蛋白, Human 是重组的 FNTA 蛋白,由 E. coli 表达,不带标签。FNTA 蛋白, Human 全长 379 个氨基酸。

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描述

FNTA protein is a key subunit in the farnesyltransferase and geranylgeranyltransferase complexes, which can transfer farnesyl or geranylgeranyl moieties to proteins with Cys-aliphatic-aliphatic-X sequence cysteine residues in proteins. This enzymatic activity contributes to post-translational protein modifications. FNTA Protein, Human is the recombinant human-derived FNTA protein, expressed by E. coli , with tag free. The total length of FNTA Protein, Human is 379 a.a., .

研究背景

FNTA 蛋白是法尼基转移酶和香叶基香叶基转移酶复合物中的重要亚基,在将法尼基或香叶基香叶基部分转移至以 C 端序列 Cys-脂肪族-脂肪族-X 为特征的蛋白质内的半胱氨酸残基方面发挥着至关重要的作用。这种酶活性有助于各种蛋白质的翻译后修饰。此外,FNTA 可能在神经肌肉接头发育中发挥积极的调节作用,通过促进 RAC1 的异戊二烯化和激活而在 MUSK 下游发挥作用。这凸显了该蛋白质参与细胞过程,对信号传导和细胞结构具有更广泛的影响。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P49354 (M1-Q379)

基因 ID

2339

蛋白结构
N-term
FNTA (M1-Q379)
Accession # P49354
C-term
同用名
FNTA; Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha; CAAX farnesyltransferase subunit alpha; FTase-alpha; Ras proteins prenyltransferase subunit alpha; Type I protein geranyl-geranyltransferase subunit alpha; GGTase-I-alpha
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FNTA Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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