1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. Fructose-bisphosphate aldolase A/ALDOA 蛋白, Human (His)

Fructose-bisphosphate aldolase A/ALDOA 蛋白, Human (His)

目录号: HY-P700265
COA 产品使用指南

果糖二磷酸醛缩酶 A (ALDOA) 是糖酵解和糖异生过程中催化 β-D-果糖 1,6-二磷酸 (FBP) 可逆转化为二丙糖磷酸的关键酶。发挥核心作用。通过介导碳水化合物代谢中的这一关键步骤,ALDOA 有助于产生细胞功能所需的能量和代谢中间体。Fructose-bisphosphate aldolase A/ALDOA 蛋白, Human (His) 是重组的 果糖二磷酸醛缩酶 A/ALDOA 蛋白,由 E. coli 表达,带有 N-6*His 标签。

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规格 价格 是否有货 数量
20 μg ¥1600 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

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  • 生物活性

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描述

Fructose-bisphosphate aldolase A (ALDOA) is a key enzyme that catalyzes the reversible conversion of β-D-fructose 1,6-bisphosphate (FBP) into two triose phosphates in glycolysis and gluconeogenesis. play a central role. By mediating this critical step in carbohydrate metabolism, ALDOA helps produce energy and metabolic intermediates necessary for cellular function. Fructose-bisphosphate aldolase A/ALDOA Protein, Human (His) is the recombinant human-derived Fructose-bisphosphate aldolase A/ALDOA protein, expressed by E. coli , with N-6*His labeled tag.

研究背景

果糖二磷酸醛缩酶 A (ALDOA) 是一种关键酶,可催化 β-D-果糖 1,6-二磷酸 (FBP) 可逆转化为两种磷酸丙糖,在糖酵解和糖异生中发挥着至关重要的作用。这种酶活性对于分解葡萄糖以产生能量以及从非碳水化合物前体合成葡萄糖是不可或缺的。除了在碳水化合物代谢中的作用之外,ALDOA 还可能充当支架蛋白,这表明其在细胞过程中除了其经典酶促功能之外还发挥着其他作用。ALDOA 在糖酵解和非糖酵解功能中的多功能性凸显了其在各种细胞过程中的重要性,并强调了其作为治疗靶点的潜在意义。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P04075 (P2-Y364)

基因 ID

226  [NCBI]

蛋白结构
N-term
6*His
Fructose-bisphosphate aldolase A/ALDOA (P2-Y364)
Accession # P04075
C-term
同用名
Lung cancer antigen NY-LU-1, Muscle-type aldolase
氨基酸序列

MPYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY

分子量

45.3 kDa

纯度

Greater than 90% as determined by SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Fructose-bisphosphate aldolase A/ALDOA Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Fructose-bisphosphate aldolase A/ALDOA Protein, Human (His)
目录号:
HY-P700265
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