Heparinase I 蛋白, B. thetaiotaomicron (His)

Heparinase I protein is an enzyme that degrades heparin and heparan sulfate and can modify extracellular matrix components. In addition to degradation, it also releases growth factors that bind to heparin, affecting the extracellular environment. Heparinase I Protein, B. thetaiotaomicron (His) is the recombinant Heparinase I protein, expressed by E. coli , with N-His labeled tag.

肝素酶 I 蛋白作为一种酶，能够降解肝素和硫酸乙酰肝素，展示其在修饰细胞外基质成分中的作用。除了其降解活性外，肝素酶 I 还参与从细胞外基质释放与肝素结合的生长因子。这种多方面的酶在细胞外环境的调节中发挥着至关重要的作用，有助于细胞与其周围基质之间的动态相互作用。肝素酶 I 靶向肝素和硫酸乙酰肝素的能力强调了其在各种生物过程中的重要性，特别是在细胞微环境中生长因子可用性和信号传导的调节中。

Measured by its ability to liberate oligosaccharides from heparin that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 15778.03 pmol/min/µg.

Others

E. coli

N-6*His

WP_011109372.1(M1-R376)

1073402

MLTAQTKNTQTLMPLTERVNVQADSARINQIIDGCWVAVGTNKPHAIQRDFTNLFDGKPSYRFELKTEDNTLEGYAKGETKGRAEFSYCYATSDDFRGLPADVYQKAQITKTVYHHGKGACPQGSSRDYEFSVYIPSSLDSNVSTIFAQWHGMPDRTLVQTPQGEVKKLTVDEFVELEKTTFFKKNVGHEKVARLDKQGNPVKDKNGKPVYKAGKPNGWLVEQGGYPPLAFGFSGGLFYIKANSDRKWLTDKDDRCNANPGKTPVMKPLTSEYKASTIAYKLPFADFPKDCWITFRVHIDWTVYGKEAETIVKPGMLDVRMDYQEQGKKVSKHIVDNEKILIGRNDEDGYYFKFGIYRVGDSTVPVCYNLAGYSER

Approximately 45 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.