IGF-I 蛋白, Salmon

IGF-I protein is similar to insulin and exerts effective growth-promoting activity as a ligand of IGF1R. Binding to the α subunit of IGF1R activates intrinsic tyrosine kinase, triggering autophosphorylation of the β subunit. IGF-I Protein, Salmon is the recombinant IGF-I protein, expressed by E. coli , with tag free. The total length of IGF-I Protein, Salmon is 70 a.a., with molecular weight of ~8 kDa.

IGF-I 蛋白在结构和功能上与胰岛素相关，具有显着更高的生长促进活性。作为 IGF1R 的配体，它与 IGF1R 的 α 亚基结合，触发内在酪氨酸激酶活性的激活。这种激活导致 β 亚基中酪氨酸残基的自磷酸化，启动一系列下游信号转导事件，从而激活 PI3K-AKT/PKB 和 Ras-MAPK 通路。IGF-I 还与整合素结合，与整合素和 IGFR1 形成三元复合物，这对 IGF1 信号传导至关重要。这种复杂的分子相互作用凸显了 IGF-I 在介导细胞反应和促进生长功能中的多方面作用。

Measure by its ability by a dose-response proliferation assay using human FDC-P1 cells. The ED50 for this effect is <15 ng/mL. The specific activity of this protein is > 6.7 × 10⁴ IU/mg. (It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.)

Others

E. coli

Tag Free

Q02815 (G45-A114)

100136741

GPETLCGAELVDTLQFVCGERGFYFSKPTGYGPSSRRSHNRGIVDECCFQSCELRRLEMYCAPVKSGKAA

Approximately 8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

< 0.2 EU/μg of protein by gel clotting method

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.