IGF-I R 蛋白, Human (His)

IGF-I receptor (IGF1R) mediates insulin-like growth factor effects, binding strongly to IGF1. Activation triggers PI3K-AKT/PKB and Ras-MAPK pathways, influencing cell survival, protein synthesis, and proliferation. IGF1R also signals through JAK/STAT, inhibiting JNK activation. Hybrid receptors show variable binding to IGF1 and insulin. IGF-I R Protein, Human (His) is the recombinant human-derived IGF-I R protein, expressed by E. coli , with N-6*His labeled tag. The total length of IGF-I R Protein, Human (His) is 169 a.a., with molecular weight of ~27 kDa.

IGF-I 受体 (IGF1R) 是一种受体酪氨酸激酶，在介导胰岛素样生长因子 1 (IGF1) 的作用中发挥着关键作用。它对 IGF1 表现出高亲和力，对 IGF2 和胰岛素 (INS) 表现出较低的亲和力。配体结合后，IGF1R 激活其激酶，导致受体自身磷酸化和各种底物的酪氨酸磷酸化，包括胰岛素受体底物 (IRS1/2)、Shc 和 14-3-3 蛋白。这会启动两条主要信号传导途径：PI3K-AKT/PKB 途径（抑制细胞凋亡并刺激蛋白质合成）和 Ras-MAPK 途径（促进细胞增殖）。磷酸化的 IRS1 可以激活 PI3K 通路，导致下游 AKT/PKB 激活，随后增强蛋白质合成和抗凋亡作用。同时，磷酸化的 IRS1 或 Shc 招募 Grb2/SOS 会激活 Ras-MAPK 通路。此外，IGF1R 通过 JAK/STAT 途径发出信号，可能有助于其转化活性。JNK激酶也可以被IGF1R激活，IGF1通过磷酸化和抑制MAP3K5/ASK1来抑制JNK激活。由 IGF1R 和 INSR 亚型组成的混合受体表现出不同的结合特征，表明对 IGF1 的高亲和力和对胰岛素的低亲和力。

Human

E. coli

N-6*His

P08069 (Y763-E931)

3480  [NCBI]

YNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTGYE

Approximately 27 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.