KRT18 蛋白, Human (HEK293, His)

The KRT18 protein is a heterotetramer with KRT8 that has multiple roles, including uptake of thrombin-antithrombin complexes and assisting in the delivery of mutated CFTR to the plasma membrane. It cooperates with KRT8 in IL-6-mediated barrier protection and interacts with PLEC isoform 1C. KRT18 Protein, Human (HEK293, His) is the recombinant human-derived KRT18 protein, expressed by HEK293 , with N-10*His labeled tag. The total length of KRT18 Protein, Human (HEK293, His) is 429 a.a., with molecular weight of ~50.8 kDa.

KRT18 蛋白在细胞过程中发挥多种作用，包括肝细胞摄取凝血酶-抗凝血酶复合物以及磷酸化时的细丝重组。它积极参与将突变的 CFTR 传递到质膜，并与 KRT8 合作实现白细胞介素 6 (IL-6) 介导的屏障保护。作为两种 I 型角蛋白和两种 II 型角蛋白的异四聚体，KRT18 与 KRT8 形成复合物，并在与 KRT8 形成异二聚体时与 PLEC 同工型 1C 相互作用。此外，KRT18 参与与凝血酶-抗凝血酶复合物的相互作用，并表现出与各种蛋白质的连接，例如 PNN、突变 CFTR、YWHAE、YWHAH、YWHAZ（磷酸化时）、DNAJB6、TCHP、TRADD、FAM83H、EPPK1、PKP1 和 PKP2。KRT18 的多方面相互作用和功能凸显了它对基本细胞活动的参与及其对控制细胞结构和信号传导的分子途径的贡献。

Human

HEK293

N-10*His

P05783 (S2-H430)

3875  [NCBI]

SFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

Approximately 50.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.