KYAT1 蛋白, Human (His)

The KYAT1 protein catalyzes the irreversible ammonia action of L-kynurenine to produce kynurenic acid (KA), which is an intermediate in the tryptophan catabolic pathway and a broad-spectrum antagonist of excitatory amino acid receptors. KYAT1 Protein, Human (His) is the recombinant human-derived KYAT1 protein, expressed by E. coli , with C-6*His labeled tag.

KYAT1 蛋白通过催化 L-色氨酸代谢物 L-犬尿氨酸的不可逆转氨作用，导致犬尿氨酸 (KA) 的形成，在细胞代谢中发挥关键作用。这种酶活性是色氨酸分解代谢途径不可或缺的一部分，其中犬尿酸充当中间体。值得注意的是，犬尿酸可作为多种受体的广谱拮抗剂，包括三种离子型兴奋性氨基酸受体。除了参与色氨酸代谢之外，KYAT1 还在特定卤代烯烃和烷烃的半胱氨酸缀合物的生物转化中发挥作用，有助于反应性代谢物的产生。此外，KYAT1 催化 L-（硒代）半胱氨酸的 S-缀合物和 Se-缀合物的 β-消除，导致 CS 或 C-Se 键的断裂。

Measured by its ability to hydrolyze 5mΜ se - methylselenopysteine that incubate at 37°C for 20 min. The specific activity is 162.351 pmol/min/μg.

Human

E. coli

C-6*His

Q16773-2 (M1-L372)

883  [NCBI]

MAKQLQARRLDGIDYNPWVEFVKLASEHDVVNLGQGFPDFPPPDFAVEAFQHAVSGDFMLNQYTKTFVIIIEPFFDCYEPMTMMAGGRPVFVSLKPGPIQNGELGSSSNWQLDPMELAGKFTSRTKALVLNTPNNPLGKVFSREELELVASLCQQHDVVCITDEVYQWMVYDGHQHISIASLPGMWERTLTIGSAGKTFSATGWKVGWVLGPDHIMKHLRTVHQNSVFHCPTQSQAAVAESFEREQLLFRQPSSYFVQFPQAMQRCRDHMIRSLQSVGLKPIIPQGSYFLITDISDFKRKMPDLPGAVDEPYDRRFVKWMIKNKGLVAIPVSIFYSVPHQKHFDHYIRFCFVKDEATLQAMDEKLRKWKVEL

Approximately 42 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCL, 500 mM NaCl, pH 8.0, 6% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.