MICA 蛋白, Human (285a.a, HEK293, His)

MICA is a ligand for the stress signaling protein and natural killer (NK) cell activating receptor KLRK1/NKG2D. Tumor cells shed the expressed MICA protein and undergo immune evasion. MICA Protein, Human (285a.a, HEK293, His) is the recombinant human-derived MICA protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MICA Protein, Human (285a.a, HEK293, His) is 285 a.a., with molecular weight of ~60.0 kDa.

MICA 是 MHC I 类链相关蛋白，是应激信号蛋白和自然杀伤 (NK) 细胞激活受体 KLRK1/NKG2D 的配体。MICA 作为应激诱导的自身抗原，被 γ δ T 细胞识别。肿瘤细胞通过脱落过度表达的 MICA、破坏 NKG2D 的生物功能而发生逃逸。MICA 高表达的 CRC 患者预后较差。在接受根治性放化疗 (CRT) 的头颈鳞状细胞癌 (HNSCC) 患者中，可溶性 MICA 相关序列 和 TGF-β1 水平持续升高标志着肿瘤进展或死亡的风险较高。

Human

HEK293

C-6*His

AAH16929.1 (E24-Q308)

100507436  [NCBI]

EPHSLRYNLTVLSWDGSVQSGFLTEVHLDGQPFLRCDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNLETKEWTMPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLKSGVVLRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSHWQ

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.