Nucleoprotein/NP 蛋白, H1N1 (I116M, AAM75159, sf9, His)

Influenza virus nucleoprotein (NP) is a structural protein that coats viral negative-strand RNA.NP proteins protect viral RNA from degradation by cellular enzymes during the viral life cycle, fit the helical structure of RNP, regulate transcription and replication of viral RNA templates in a histone-like manner, and induce immunosuppression during infection.Nucleoprotein/NP Protein, H1N1 (I116M, AAM75159, sf9, His) is the recombinant Virus-derived Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag.

流感病毒核蛋白 (NP) 是一种包衣病毒负链 RNA 的结构蛋白。NP 是物种特异性的主要决定因素之一。NP 基因在多大程度上可以通过重组跨越物种屏障，并通过突变适应新的宿主。NP 蛋白在病毒生命周期中保护病毒 RNA 不被细胞酶降解、拟合 RNP 的螺旋结构、以组蛋白样方式调节病毒 RNA 模板的转录和复制以及在感染时诱导免疫抑制 (抑制效应细胞因子合成和 FcR 药物信号传导)。NP 是未来疫苗开发的一个非常有前途的目标。

Virus

Sf9 insect cells

C-His

AAM75159 (M1-G490, I116M)

/

Approximately 50 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 60 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.