1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E1 Enzymes
  5. PDHA1 蛋白, Human (GST)

PDHA1蛋白是丙酮酸脱氢酶复合物的重要组成部分,负责协调丙酮酸向乙酰辅酶A和CO2的转化。它在糖酵解和三羧酸 (TCA) 循环之间建立了重要联系,确保代谢途径的无缝整合。PDHA1 蛋白, Human (GST) 是重组的 PDHA1 蛋白,由 E. coli 表达,带有 N-GST 标签。

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描述

The PDHA1 protein is an important component of the pyruvate dehydrogenase complex and is responsible for coordinating the conversion of pyruvate to acetyl-CoA and CO2. It establishes an important link between glycolysis and the tricarboxylic acid (TCA) cycle, ensuring seamless integration of metabolic pathways. PDHA1 Protein, Human (GST) is the recombinant human-derived PDHA1 protein, expressed by E. coli, with N-GST labeled tag.

研究背景

PDHA1 蛋白是丙酮酸脱氢酶复合物的重要组成部分,在协调丙酮酸全面转化为乙酰辅酶 A 和 CO2 方面发挥着关键作用。这种酶促过程在糖酵解途径和三羧酸 (TCA) 循环之间建立了基本联系,促进代谢途径的无缝整合。PDHA1 的催化功能对于丙酮酸的有效转化至关重要,确保碳底物流入 TCA 循环,以实现细胞内随后的能量生成和代谢稳态。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

P08559 (A31-S390)

基因 ID

5160

蛋白结构
N-term
GST
A31-S390
Accession # P08559
C-term
蛋白长度

Partial

同用名
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1; PHE1A
氨基酸序列

ANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

预测分子量
67.6 kDa
纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PDHA1 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PDHA1 Protein, Human (GST)
目录号:
HY-P704073
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