Pepsinogen A4/PGA4 蛋白, Human (HEK293, His)

Pepsinogen A4/PGA4 protein, known for broad substrate specificity, prefers cleaving phenylalanine and leucine bonds but also shows the ability to cleave various other bonds to varying extents. Pepsinogen A4/PGA4 Protein, Human (HEK293, His) is the recombinant human-derived Pepsinogen A4/PGA4 protein, expressed by HEK293 , with C-His labeled tag.

胃蛋白酶原 A4/PGA4 蛋白表现出显着广泛的底物特异性，优先裂解涉及苯丙氨酸和亮氨酸的键。尽管存在这种偏好，但它表现出在不同程度上裂解许多其他类型键的能力。

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is 69099.036 pmol/min/μg that incubate at room temperature for 15 minutes.

Human

HEK293

C-6*His

P0DJD7 (I16-A388)

643847  [NCBI]

IMYKVPLIRKKSLRRTLSERGLLKDFLKKHNLNPARKYFPQWEAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVVFDTGSSNLWVPSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLSADDQSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITMNGEAIACAEGCQAIVDTGTSLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPSAYILQSEGSCISGFQGMNLPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA

Approximately 45 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.