PRKACB 蛋白, Human (sf9)

PRKACB Protein, Human (sf9) is the recombinant human-derived PRKACB, expressed by Sf9 insect cells , with tag Free labeled tag. ,

PRKACB 是一种介导 cAMP 依赖性信号传导的蛋白激酶，其信号通路由 GPCRs 受体结合触发。PKA 的激活调控多种细胞过程，包括细胞增殖、细胞周期、分化、微管动力学、染色质凝集与解凝、核膜解体与重组，以及细胞内运输机制和离子通量的调控。PRKACB 通过磷酸化 PJA2 来调控其调节亚基的区域化池丰度，PJA2 能结合并泛素化这些亚基，导致其后续的蛋白水解。此外，PRKACB 磷酸化 GPKOW，从而调控其与 RNA 的结合能力。类似功能 (by similar)：PRKACB 还作为 mTORC1 的负调控因子，通过磷酸化 RPTOR 发挥作用。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. When the protein concentration is 0.025 nM, the conversion rate is about 25%.

Human

Sf9 insect cells

Tag Free

P22694-1 (G2-F351)

5567

Full Length of Isoform-1 Mature Protein

40.6 KDa

Greater than 80 % as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.